| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
CHARACTERIZATION SERIES |
a Department of Neurology, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts, USA
b Center for Neuroregeneration Research, McLean Hospital, Harvard Medical School, Belmont, Massachusetts, USA
c Neuroscience Research Institute, University of California-Santa Barbara, Santa Barbara, California, USA
Key Words. Embryonic stem cells • MicroRNA array • MicroRNA • Neurogenesis
Correspondence: Anna M. Krichevsky, Ph.D., 4 Blackfan Circle, HIM 760, Boston, Massachusetts 02115, USA. Telephone: 617-525-5195; Fax: 617-525-5305; e-mail: krichevsky{at}cnd.bwh.harvard.edu; and Kai-C. Sonntag, M.D., Ph.D., 115 Mill Street, Belmont, Massachusetts 02478, USA. Telephone: 617-855-3138, Fax: 617-855-3284; e-mail: kai.sonntag{at}mclean.harvard.edu
Received September 8, 2005;
accepted for publication December 7, 2005.
MicroRNAs (miRNAs) are recently discovered small non-coding transcripts with a broad spectrum of functions described mostly in invertebrates. As post-transcriptional regulators of gene expression, miRNAs trigger target mRNA degradation or translational repression. Although hundreds of miRNAs have been cloned from a variety of mammalian tissues and cells and multiple mRNA targets have been predicted, little is known about their functions. So far, a role of miRNA has only been described in hematopoietic, adipocytic, and muscle differentiation; regulation of insulin secretion; and potentially regulation of cancer growth. Here, we describe miRNA expression profiling in mouse embryonic stem (ES) cell– derived neurogenesis in vitro and show that a number of miRNAs are simultaneously co-induced during differentiation of neural progenitor cells to neurons and astrocytes. There was a clear correlation between miRNA expression profiles in ES cell– derived neurogenesis in vitro and in embryonal neurogenesis in vivo. Using both gain-of-function and loss-of-function approaches, we demonstrate that brain-specific miR-124a and miR-9 molecules affect neural lineage differentiation in the ES cell– derived cultures. In addition, we provide evidence that signal transducer and activator of transcription (STAT) 3, a member of the STAT family pathway, is involved in the function of these miRNAs. We conclude that distinct miRNAs play a functional role in the determination of neural fates in ES cell differentiation.
This article has been cited by other articles:
|
|
 |
|
  A. N. Packer, Y. Xing, S. Q. Harper, L. Jones, and B. L. Davidson The Bifunctional microRNA miR-9/miR-9* Regulates REST and CoREST and Is Downregulated in Huntington's Disease J. Neurosci., December 31, 2008; 28(53): 14341 - 14346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. De Pietri Tonelli, J. N. Pulvers, C. Haffner, E. P. Murchison, G. J. Hannon, and W. B. Huttner miRNAs are essential for survival and differentiation of newborn neurons but not for expansion of neural progenitors during early neurogenesis in the mouse embryonic neocortex Development, December 1, 2008; 135(23): 3911 - 3921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Thum, D. Catalucci, and J. Bauersachs MicroRNAs: novel regulators in cardiac development and disease Cardiovasc Res, September 1, 2008; 79(4): 562 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Chen, F. Song, G. A. Calin, Q. Wei, X. Hao, and W. Zhang Polymorphisms in microRNA targets: a gold mine for molecular epidemiology Carcinogenesis, July 1, 2008; 29(7): 1306 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. W. Yeo, N. Coufal, S. Aigner, B. Winner, J. A. Scolnick, M. C.N. Marchetto, A. R. Muotri, C. Carson, and F. H. Gage Multiple layers of molecular controls modulate self-renewal and neuronal lineage specification of embryonic stem cells Hum. Mol. Genet., April 15, 2008; 17(R1): R67 - R75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Lakshmipathy and R. P. Hart Concise Review: MicroRNA Expression in Multipotent Mesenchymal Stromal Cells Stem Cells, February 1, 2008; 26(2): 356 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M.-S. Tay, W.-L. Tam, Y.-S. Ang, P. M. Gaughwin, H. Yang, W. Wang, R. Liu, J. George, H.-H. Ng, R. J. Perera, et al. MicroRNA-134 Modulates the Differentiation of Mouse Embryonic Stem Cells, Where It Causes Post-Transcriptional Attenuation of Nanog and LRH1 Stem Cells, January 1, 2008; 26(1): 17 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Brunlid, J. Pruszak, B. Holmes, O. Isacson, and K.-C. Sonntag Immature and Neurally Differentiated Mouse Embryonic Stem Cells Do Not Express a Functional Fas/Fas Ligand System Stem Cells, October 1, 2007; 25(10): 2551 - 2558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang, J. E. Dahlberg, and W. Tam MicroRNAs in Tumorigenesis: A Primer Am. J. Pathol., September 1, 2007; 171(3): 728 - 738. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pruszak, K.-C. Sonntag, M. H. Aung, R. Sanchez-Pernaute, and O. Isacson Markers and Methods for Cell Sorting of Human Embryonic Stem Cell-Derived Neural Cell Populations Stem Cells, September 1, 2007; 25(9): 2257 - 2268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wu, J. Xu, Z. P. Pang, W. Ge, K. J. Kim, B. Blanchi, C. Chen, T. C. Sudhof, and Y. E. Sun Integrative genomic and functional analyses reveal neuronal subtype differentiation bias in human embryonic stem cell lines PNAS, August 21, 2007; 104(34): 13821 - 13826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sathyan, H. B. Golden, and R. C. Miranda Competing Interactions between Micro-RNAs Determine Neural Progenitor Survival and Proliferation after Ethanol Exposure: Evidence from an Ex Vivo Model of the Fetal Cerebral Cortical Neuroepithelium J. Neurosci., August 8, 2007; 27(32): 8546 - 8557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Mehler and J. S. Mattick Noncoding RNAs and RNA Editing in Brain Development, Functional Diversification, and Neurological Disease Physiol Rev, July 1, 2007; 87(3): 799 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Suarez, C. Fernandez-Hernando, J. S. Pober, and W. C. Sessa Dicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells Circ. Res., April 27, 2007; 100(8): 1164 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, F. Wang, J.-A Lee, and F.-B. Gao MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila Genes & Dev., October 15, 2006; 20(20): 2793 - 2805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Rao One Successful Series Begets Another Stem Cells, October 1, 2006; 24(10): 2160 - 2161. [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Mehler and J. S. Mattick Non-coding RNAs in the nervous system J. Physiol., September 1, 2006; 575(2): 333 - 341. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
