HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2005-0363v1 24/5/1265    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, Z. Articles by Li, D.-Q. Search for Related Content PubMed PubMed Citation Articles by Chen, Z. Articles by Li, D.-Q.

TISSUE-SPECIFIC STEM CELLS

Gap Junction Protein Connexin 43 Serves as a Negative Marker for a Stem Cell-Containing Population of Human Limbal Epithelial Cells

^a Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, USA;
^b Department of Medical Biochemistry and Immunology, Cardiff University Medical School and The Wales Heart Research Institute, Cardiff, Wales, United Kingdom

Key Words. Connexin 43 • Cornea • Limbus • Epithelia • Stem cells

Correspondence: De-Quan Li, M.D., Ph.D., Ocular Surface Center, Cullen Eye Institute, Department of Ophthalmology, Baylor College of Medicine, 6565 Fannin Street, NC-205, Houston, Texas 77030, USA. Telephone: 713-798-1123; Fax: 713-798-1457; e-mail: dequanl{at}bcm.tmc.edu

Received August 3, 2005; accepted for publication January 7, 2006.
This study evaluated whether the gap junction protein connexin (Cx) 43 could serve as a negative cell surface marker for human corneal epithelial stem cells. Cx43 expression was evaluated in corneo-limbal tissue and primary limbal epithelial cultures. Immunofluorescent staining and laser scanning confocal microscopy showed that Cx43 was strongly expressed in the corneal and limbal suprabasal epithelial cells, but the basal cells of the limbal epithelium were negative. Cx43 antibody stained mainly large cells but not small cells in primary limbal epithelial cultures. As determined by semiquantitative reverse transcription polymerase chain reaction (PCR) and real-time PCR, Cx43 mRNA was more abundant in the corneal than limbal epithelia, and it was expressed in higher levels in mature limbal epithelial cultures. Using GAP11, a rabbit polyclonal antibody against the Cx32 extracellular loop 2 (151–187), a sequence that is highly homologous in Cx43, the Cx43^dim and Cx43^bright cells were selected from primary limbal epithelial cultures by fluorescence-activated cell sorting and were evaluated for stem cell properties. These Cx43^dim and Cx43^bright cells were confirmed by their expression levels of Cx43 protein and mRNA. The Cx43^dim cells were found to contain higher percentages of slow-cycling bromodeoxyuridine (BrdU)-label retaining cells and the cells that were positive for stem cell-associated markers p63, ABCG2, and integrin ß1 and negative for differentiation markers K3 and involucrin. The Cx43^dim cells possessed a greater proliferative potential than Cx43^bright cells and nonfractionated cells as evaluated by BrdU incorporation, colony-forming efficiency, and growth capacity. Our findings suggest that human limbal basal cells do not express connexin 43, which could serve as a negative cell surface marker for the stem cell-containing population of human limbal epithelial cells.

This article has been cited by other articles:

				H Qi, D-Q Li, F Bian, E Y Chuang, D B Jones, and S C Pflugfelder Expression of glial cell-derived neurotrophic factor and its receptor in the stem-cell-containing human limbal epithelium Br. J. Ophthalmol., September 1, 2008; 92(9): 1269 - 1274. [Abstract] [Full Text] [PDF]

				Z. Chen, L. Tong, Z. Li, K.-C. Yoon, H. Qi, W. Farley, D.-Q. Li, and S. C. Pflugfelder Hyperosmolarity-Induced Cornification of Human Corneal Epithelial Cells Is Regulated by JNK MAPK Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 539 - 549. [Abstract] [Full Text] [PDF]

				S. Raeder, T. P. Utheim, O. A. Utheim, B. Nicolaissen, B. Roald, Y. Cai, K. Haug, A. Kvalheim, E. B. Messelt, L. Drolsum, et al. Effects of Organ Culture and Optisol-GS Storage on Structural Integrity, Phenotypes, and Apoptosis in Cultured Corneal Epithelium Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5484 - 5493. [Abstract] [Full Text] [PDF]

				Y.-T. Chen, W. Li, Y. Hayashida, H. He, S.-Y. Chen, D. Y. Tseng, A. Kheirkhah, and S. C. G. Tseng Human Amniotic Epithelial Cells as Novel Feeder Layers for Promoting Ex Vivo Expansion of Limbal Epithelial Progenitor Cells Stem Cells, August 1, 2007; 25(8): 1995 - 2005. [Abstract] [Full Text] [PDF]

				V. A Shanmuganathan, T. Foster, B. B Kulkarni, A. Hopkinson, T. Gray, D. G Powe, J. Lowe, and H. S Dua Morphological characteristics of the limbal epithelial crypt Br. J. Ophthalmol., April 1, 2007; 91(4): 514 - 519. [Abstract] [Full Text] [PDF]