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TISSUE-SPECIFIC STEM CELLS

Dynamic Interactions of Chromatin-Related Mesenchymal Modulator, a Chromodomain Helicase-DNA-Binding Protein, with Promoters in Osteoprogenitors

Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

Key Words. Differentiation • CHD • Promoters • Progenitors

Correspondence: Dafna Benayahu, Ph.D., Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 66978, Israel. Telephone: 972-3-640-6187; Fax: 972-3-640-7432; e-mail: dafnab{at}post.tau.ac.il

Received July 6, 2005; accepted for publication December 21, 2005.
The newly identified protein chromatin-related mesenchymal modulator (CReMM) is expressed by marrow stromal progenitors in vivo and ex vivo. CReMM belongs to a recently identified subgroup of chromodomain helicase-DNA-binding proteins composed of multiple domains including chromodomains, SNF2/ATPase, helicase-C domain, SANT, and A/T-hook-DNA binding domain. Chromatin immunoprecipitation assay was applied to follow the dynamics of CReMM binding to A/T-rich regions on promoters of genes that play a role in osteoblast maturation. CReMM interaction with BMP4 and biglycan promoters in the marrow stromal cells was challenged with transforming growth factor-ß. Treatment with 17ß-estradiol enhanced the binding to estrogen receptor and abolished binding to the prolactin receptor promoters; CReMM interaction with osteocalcin promoter was identified constantly. CReMM binding to the analyzed endogenous promoters suggests its direct role in the transcriptional program activated during osteogenic cell differentiation, which may be a useful tool for following the molecular mechanism of the "stemness" of mesenchymal cells.