Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 2, 2006
Stem Cells Vol. 24 No. 5 May 2006, pp. 1370 -1380
doi:10.1634/stemcells.2005-0340; www.StemCells.com
© 2006 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-0340v1
24/5/1370    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tian, X.
Right arrow Articles by Kaufman, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tian, X.
Right arrow Articles by Kaufman, D. S.

EMBRYONIC STEM CELLS

Hematopoietic Engraftment of Human Embryonic Stem Cell-Derived Cells Is Regulated by Recipient Innate Immunity

Xinghui Tian, Petter S. Woll, Julie K. Morris, Jonathan L. Linehan, Dan S. Kaufman

Stem Cell Institute, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA

Key Words. Hematopoiesis • Human embryonic stem cells • Natural killer cells • Transplantation

Correspondence: Dan S. Kaufman, M.D., Ph.D., Stem Cell Institute, Translational Research Facility, 2001 6th St. SE, Mail Code 2873, Minneapolis, Minnesota 55455, USA. Telephone: 612-624-0922; Fax: 612-624-2436; e-mail: kaufm020{at}umn.edu

Received July 27, 2005; accepted for publication January 22, 2006.
Human embryonic stem cells (hESCs) provide an important means to characterize early stages of hematopoietic development. However, the in vivo potential of hESC-derived hematopoietic cells has not been well defined. We demonstrate that hESC-derived cells are capable of long-term hematopoietic engraftment when transplanted into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice. Human CD45+ and CD34+ cells are identified in the mouse bone marrow (BM) more than 3 months after injection of hESCs that were allowed to differentiate on S17 stromal cells for 7–24 days. Secondary engraftment studies further confirm long-term repopulating cells derived from hESCs. We also evaluated two mechanisms that may inhibit engraftment: host immunity and requirement for homing to BM. Treatment with anti-ASGM1 antiserum that primarily acts by depletion of natural killer cells in transplanted mice leads to improved engraftment, likely due to low levels of HLA class I expressed on hESCs and CD34+ cells derived from hESCs. Intra-BM injection also provided stable engraftment, with hematopoietic cells identified in both the injected and contra-lateral femur. Importantly, no teratomas are evident in animals injected with differentiated hESCs. These results demonstrate that SCID-repopulating cells, a close surrogate for hematopoietic stem cells, can be derived from hESCs. Moreover, both adaptive and innate immune effector cells may be barriers to engraftment of these cells.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
Z. Su, C. Frye, K.-M. Bae, V. Kelley, and J. Vieweg
Differentiation of Human Embryonic Stem Cells into Immunostimulatory Dendritic Cells under Feeder-Free Culture Conditions
Clin. Cancer Res., October 1, 2008; 14(19): 6207 - 6217.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. Ji, K. Vijayaragavan, M. Bosse, K. Weisel, and M. Bhatia
OP9 Stroma Augments Survival of Hematopoietic Precursors and Progenitors During Hematopoietic Differentiation from Human Embryonic Stem Cells
Stem Cells, October 1, 2008; 26(10): 2485 - 2495.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. H. Martin, P. S. Woll, Z. Ni, J. C. Zuniga-Pflucker, and D. S. Kaufman
Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood-derived hematopoietic progenitor cells
Blood, October 1, 2008; 112(7): 2730 - 2737.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
C. M. Cameron, F. Harding, W.-S. Hu, and D. S. Kaufman
Activation of Hypoxic Response in Human Embryonic Stem Cell-Derived Embryoid Bodies
Experimental Biology and Medicine, August 1, 2008; 233(8): 1044 - 1057.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. S. Woll, J. K. Morris, M. S. Painschab, R. K. Marcus, A. D. Kohn, T. L. Biechele, R. T. Moon, and D. S. Kaufman
Wnt signaling promotes hematoendothelial cell development from human embryonic stem cells
Blood, January 1, 2008; 111(1): 122 - 131.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G. de Wert, I. Liebaers, and H. Van de Velde
The Future (R)evolution of Preimplantation Genetic Diagnosis/Human Leukocyte Antigen Testing: Ethical Reflections
Stem Cells, September 1, 2007; 25(9): 2167 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
M. Bhatia
Hematopoietic Development from Human Embryonic Stem Cells
Hematology, January 1, 2007; 2007(1): 11 - 16.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. E. Bouhassira
34 + 43 = early human blood lineage
Blood, September 15, 2006; 108(6): 1787 - 1788.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS

Copyright © 2006 by AlphaMed Press.