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STEM CELL GENETICS AND GENOMICS

Gene Expression Profiles in Murine Hematopoietic Stem Cells Revisited: Analysis of cDNA Libraries Reveals High Levels of Translational and Metabolic Activities

^aClinic of Hematology, University Hospital of Essen, Essen, Germany;
^bLeukaemia Foundation of Queensland Laboratory, Queensland Institute of Medical Research, Brisbane, Queensland, Australia;
^cInstitute of Cell Biology, University Hospital of Essen, Essen, Germany;
^dInstitute for Medical Informatics, Biometry and Epidemiology, University Hospital of Essen, Essen, Germany;
^eInstitute of Cellular and Organismic Biology/Genomics Research Center, Academia Sinica, Taipei, Taiwan

Key Words. Gene expression • Quiescence • Mouse • Microarray • Hematopoietic stem cells

Correspondence: Andreas Hüttmann, M.D.,Klinik für Hämatologie, Zentrum für Innere Medizin, Universitätsklinikum, Hufelandstr. 55, 45122 Essen, Germany.Telephone: ++49 201 723 2417;Fax: ++49 201 723 5928;email: andreas.huettmann{at}uni-essen.de

Received on October 1, 2005; accepted for publication on March 22, 2006.

First published online in STEM CELLS EXPRESS  March 30, 2006.

Gene expression studies from hematopoietic stem cell (HSC) populations purified to variable degrees have defined a set of stemness genes. Unexpectedly, results also hinted toward a HSC chromatin poised in a wide-open state. With the aim of providing a robust tool for further studies into the molecular biology of HSCs, the studies herein describe the construction and comparative molecular analysis of -phage cDNA libraries from highly purified HSCs that retained their long-term repopulating activities (long-term HSCs [LT-HSCs]) and from short-term repopulating HSCs that were largely depleted of these activities. Microarray analysis of the libraries confirmed the previous results but also revealed an unforeseen preferential expression of translation- and metabolism-associated genes in the LT-HSCs. Therefore, these data indicate that HSCs are quiescent only in regard of proliferative activities but are in a state of readiness to provide the metabolic and translational activities required after induction of proliferation and exit from the HSC pool.

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