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TISSUE-SPECIFIC STEM CELLS

In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Hepatocyte-Like Cells

^aCathay Medical Research Institute,
^eDepartment of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan;
^bDepartment of Medicine, Fu-Jen Catholic University, Taipei, Taiwan;
^cStem Cell Research Center, National Health Research Institutes, Taipei, Taiwan;
^dDepartment of Forensic Medicine, National Taiwan University, Taipei, Taiwan

Key Words. Placenta • Differentiation • Hepatocyte • Regenerative medicine

Correspondence: Hsing-I Huang, Ph.D., Cathay Medical Research Institute, Cathay General Hospital, No. 32, Ln 160, Jian-Cheng Road, 22141, Hsi-Chih, Taipei, Taiwan, R.O.C. Telephone: 886-2-26907965, ext. 2312; Fax: 886-2-26907963; email: hihuang{at}cgh.org.tw

Received on October 20, 2005; accepted for publication on March 15, 2006.

First published online in STEM CELLS EXPRESS  March 15, 2006.

Multipotent cells isolated from human term placenta (placenta-derived multipotent cells [PDMCs]) have been known to be able to differentiate into mesodermal lineage cells, including adipocytes and osteoclasts. The low infection rate and young age of placenta compared with other tissue origins of adult stem cells make theses cells attractive target for cell-based therapy. However, the differentiation potential of PDMCs toward hepatic cells has not been evaluated yet. In this study, we cultivated PDMCs with hepatic differentiation medium to evaluate the ability of these cells in differentiating toward hepatic cells. After treatment, the morphologies of differentiated PDMCs changed to polygonal epithelial cell-like. The differentiated cells not only show the hepatocyte-like morphologies but also express hepatocyte-specific markers, including albumin and cytokeratin 18. The bioactivity assays revealed that these hepatocyte-like cells could uptake lipoprotein and store glycogen. Furthermore, the addition of rifampicin increased the gene expression of CYP3A4, which is similar with the activities of human liver cells. According to our previous results, PDMCs were capable of differentiating into mesodermal and ectodermal lineage cells. Our results indicate that PDMCs can differentiate into three germ layer cells, which is similar to embryonic stem cells. In conclusion, placenta might be an easily accessible source for progenitor cells that are capable of differentiating toward hepatocyte-like cells in vitro.

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