Stem Cells
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Stem Cells Vol. 24 No. 7 July 2006, pp. 1789 -1797
doi:10.1634/stemcells.2005-0492; www.StemCells.com
© 2006 AlphaMed Press

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TRANSLATIONAL AND CLINICAL RESEARCH

Granulocyte Colony-Stimulating Factor Mobilizes More Dendritic Cell Subsets Than Granulocyte-Macrophage Colony-Stimulating Factor with No Polarization of Dendritic Cell Subsets in Normal Donors

Paul J. Shaughnessya, Carlos Bachiera, Charles F. LeMaistrea, Cagla Akayb, Brad H. Pollockb, Yair Gazittb

aTexas Transplant Institute, San Antonio, Texas, USA;
bUniversity of Texas Health Science Center at San Antonio, San Antonio, Texas, USA

Key Words. Granulocyte colony-stimulating factor • Granulocyte-macrophage colony-stimulating factor • Graft versus host disease • Dentritic cells • Mobilization

Correspondence: Paul J. Shaughnessy, M.D., 8201 Ewing Halsell, Suite 280, San Antonio, Texas 78229, USA. Telephone: 210-575-8500; Fax: 210-575-8506; email: Paul.Shaughnessy{at}MHShealth.com

Received on October 8, 2005; accepted for publication on March 13, 2006.


Dendritic cells (DCs) are effective antigen-presenting cells. We hypothesized that increasing the DC populations in donor lymphocyte infusions (DLIs) may augment the graft versus malignancy effect, particularly if granulocyte-macrophage colony-stimulating factor (GM-CSF) mobilization resulted in increased precursor dendritic cell (pDC) 1 cells. Mature DCs, pDC1 cells, pDC2 cells, and CD34+ cells from the same donor were compared after granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood stem cell collections and GM-CSF mobilized DLI collections. Mobilization with G-CSF resulted in up to a 10-fold larger number of CD34+ cells per kg and a 3–5-fold larger number of mature DCs, pDC1 cells, and pDC2 cells within the same donor compared with GM-CSF. The ratio of pDC1 to pDC2 in each donor remained constant with either cytokine. In this small sample of normal donors, it appears that G-CSF mobilizes more CD34+ cells, mature DCs, pDC1 cells, and pDC2 cells within the same donor than does GM-CSF, with no significant polarization by G-CSF or GM-CSF for either pDC1 or pDC2 cells.




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