| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
TRANSLATIONAL AND CLINICAL RESEARCH |
Departments of aInternal Medicine and
bLaboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA
Key Words. Stem cell plasticity • Lung injury • In vivo • Mice
Correspondence: Erica Herzog, M.D., Ph.D., Department of Medicine, Pulmonary and Critical Care Division, 333 Cedar St. TAC 441-S, New Haven, Connecticut 06520, USA. Telephone: 203-785-3627; Fax: 203-785-3826; e-mail: erica.herzog{at}yale.edu
Received November 22, 2005;
accepted for publication May 1, 2006.
Bone marrow-derived cells (BMDCs) can adopt an epithelial phenotype in the lung following bone marrow transplantation (BMT). This phenomenon has been assumed to result from the lung injury that occurs with myeloablative radiation. To date, no study has related the degree of epithelial chimerism following bone marrow transplantation to the lung damage induced by preconditioning for BMT. Such a goal is crucial to understanding the local host factors that promote the engraftment of BMDCs as lung epithelia. We undertook this aim by performing sex-mismatched bone marrow transplantation using a variety of preconditioning regimens and comparing measurements of lung injury (bronchoalveolar lavage [BAL] cell count, alveolar-capillary leak assayed by BAL protein levels, and terminal deoxynucleotidyl transferase dUTP nick-end labeling analysis on epithelial cells) with rigorous methods to quantify bone marrow-derived lung epithelia (costaining for epithelial and donor markers on tissue sections and isolated lung epithelia in recipient mice). We found that only at doses that induced lung injury could marrow derived lung epithelium be identified following BMT. With irradiation doses less than 1,000 centigray (cGy), there was little to no apparent injury to the lung, and there were no marrow-derived pneumocytes despite high levels of hematopoietic chimerism. In contrast, 4 days after either split or single-dose 1,000 cGy irradiation, nearly 15% of lung epithelia were apoptotic, and with this dose, marrow-derived type II pneumocytes (0.2%) were present at 28 days. These data indicate a critical relationship between lung injury and the phenotypic change from BMDCs to lung epithelial cells.
This article has been cited by other articles:
|
|
 |
|
  D. S. Krause Bone Marrow-derived Lung Epithelial Cells Proceedings of the ATS, August 15, 2008; 5(6): 699 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Rippon, S. Lane, M. Qin, N.-S. Ismail, M. R. Wilson, M. Takata, and A. E. Bishop Embryonic Stem Cells as a Source of Pulmonary Epithelium In Vitro and In Vivo Proceedings of the ATS, August 15, 2008; 5(6): 717 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Rodman Proof-of-Concept Trials for Lung Stem Cell Therapy Proceedings of the ATS, August 15, 2008; 5(6): 731 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Liebler, C. Lutzko, A. Banfalvi, D. Senadheera, N. Aghamohammadi, E. D. Crandall, and Z. Borok Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injury Am J Physiol Lung Cell Mol Physiol, August 1, 2008; 295(2): L285 - L292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF]
|
|
|
|
|
|
D. S. Krause Bone Marrow-derived Cells and Stem Cells in Lung Repair Proceedings of the ATS, April 15, 2008; 5(3): 323 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Sueblinvong, R. Loi, P. L. Eisenhauer, I. M. Bernstein, B. T. Suratt, J. L. Spees, and D. J. Weiss Derivation of Lung Epithelium from Human Cord Blood-derived Mesenchymal Stem Cells Am. J. Respir. Crit. Care Med., April 1, 2008; 177(7): 701 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Reese, J. C. Roth, and S. L. Gerson Bone Marrow-Derived Cells Exhibiting Lung Epithelial Cell Characteristics Are Enriched In Vivo Using Methylguanine DNA Methyltransferase-Mediated Drug Resistance Stem Cells, March 1, 2008; 26(3): 675 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Levis, R. Loi, K. J. Butnor, P. Vacek, C. Steele, B. T. Mossman, and D. J. Weiss Decreased Asbestos-Induced Lung Inflammation and Fibrosis after Radiation and Bone Marrow Transplant Am. J. Respir. Cell Mol. Biol., January 1, 2008; 38(1): 16 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Wong, A. E. Dutly, A. Sacher, H. Lee, D. M. Hwang, M. Liu, S. Keshavjee, J. Hu, and T. K. Waddell Targeted cell replacement with bone marrow cells for airway epithelial regeneration Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L740 - L752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Aliotta, F. M. Sanchez-Guijo, G. J. Dooner, K. W. Johnson, M. S. Dooner, K. A. Greer, D. Greer, J. Pimentel, L. M. Kolankiewicz, N. Puente, et al. Alteration of Marrow Cell Gene Expression, Protein Production, and Engraftment into Lung by Lung-Derived Microvesicles: A Novel Mechanism for Phenotype Modulation Stem Cells, September 1, 2007; 25(9): 2245 - 2256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Herzog, J. Van Arnam, B. Hu, J. Zhang, Q. Chen, A. M. Haberman, and D. S. Krause Lung-specific nuclear reprogramming is accompanied by heterokaryon formation and Y chromosome loss following bone marrow transplantation and secondary inflammation FASEB J, August 1, 2007; 21(10): 2592 - 2601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Du and H. S. Taylor Contribution of Bone Marrow-Derived Stem Cells to Endometrium and Endometriosis Stem Cells, August 1, 2007; 25(8): 2082 - 2086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aguilar, E. Nye, J. Chan, M. Loebinger, B. Spencer-Dene, N. Fisk, G. Stamp, D. Bonnet, and S. M. Janes Murine but Not Human Mesenchymal Stem Cells Generate Osteosarcoma-Like Lesions in the Lung Stem Cells, June 1, 2007; 25(6): 1586 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Viswanathan, R. G. Painter, N. A. Lanson Jr., and G. Wang Functional Expression of N-Formyl Peptide Receptors in Human Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells, May 1, 2007; 25(5): 1263 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Herzog and D. S. Krause Engraftment of Marrow-derived Epithelial Cells: The Role of Fusion Proceedings of the ATS, November 1, 2006; 3(8): 691 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Bruscia, E. C. Ziegler, J. E. Price, S. Weiner, M. E. Egan, and D. S. Krause Engraftment of Donor-Derived Epithelial Cells in Multiple Organs Following Bone Marrow Transplantation into Newborn Mice Stem Cells, October 1, 2006; 24(10): 2299 - 2308. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
