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EMBRYONIC STEM CELLS

Tal1/Scl Gene Transduction Using a Lentiviral Vector Stimulates Highly Efficient Hematopoietic Cell Differentiation from Common Marmoset (Callithrix jacchus) Embryonic Stem Cells

^aDepartment of Molecular Genetics, Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan;
^bDepartment of Biomedical Science, Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan;
^cCell Bank, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan;
^dDivision of Molecular Therapy, Institute of Medical Science, University of Tokyo, Tokyo, Japan

Key Words. CD34⁺ cells • Transcription factor tal1/scl • Lentiviral vector • Hematopoietic cells • Gene transfer Embryonic stem cell • Embryoid body

Correspondence: Kenzaburo Tani, M.D., Ph.D., Department of Molecular Genetics, Division of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Telephone: 81-92-642-6434; Fax: 81-92-642-6444; e-mail: taniken{at}bioreg.kyushu-u.ac.jp

Received on October 17, 2005; accepted for publication on May 9, 2006.

First published online in STEM CELLS EXPRESS  May 25, 2006.


The development of embryonic stem cell (ESC) therapies requires the establishment of efficient methods to differentiate ESCs into specific cell lineages. Here, we report the in vitro differentiation of common marmoset (CM) (Callithrix jacchus) ESCs into hematopoietic cells after exogenous gene transfer using vesicular stomatitis virus-glycoprotein-pseudotyped lentiviral vectors. We transduced hematopoietic genes, including tal1/scl, gata1, gata2, hoxB4, and lhx2, into CM ESCs. By immunochemical and morphological analyses, we demonstrated that overexpression of tal1/scl, but not the remaining genes, dramatically increased hematopoiesis of CM ESCs, resulting in multiple blood-cell lineages. Furthermore, flow cytometric analysis demonstrated that CD34, a hematopoietic stem/progenitor cell marker, was highly expressed in tal1/scl-overexpressing embryoid body cells. Similar results were obtained from three independent CM ESC lines. These results suggest that transduction of exogenous tal1/scl cDNA into ESCs is a promising method to induce the efficient differentiation of CM ESCs into hematopoietic stem/progenitor cells.

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