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TISSUE-SPECIFIC STEM CELLS

Integrins Are Markers of Human Neural Stem Cells

^aDepartment of Pathology and
^bCambridge Centre for Brain Repair, University of Cambridge, Cambridge, United Kingdom;
^cLaboratory of Neurosciences, National Institute on Aging, Baltimore, Maryland, USA

Key Words. Fluorescence-activated cell sorting • Neurosphere • Prominin • Laminin • Extracellular matrix

Correspondence: Charles ffrench-Constant, FRCP, Ph.D., Department of Pathology, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Telephone: +44-1223-333723; Fax: +44-1223-333346; e-mail: cfc{at}mole.bio.cam.ac.uk

Received November 28, 2005; accepted for publication May 3, 2006.


The identification of markers for the isolation of human neural stem cells (hNSCs) is essential for studies of their biology and therapeutic applications. This study investigated expression of the integrin receptor family by hNSCs as potential markers. Selection of 6^hi or ß1^hi cells by fluorescence-activated cell sorting led to an enrichment of human neural precursors, as shown by both neurosphere forming assays and increased expression of prominin-1, sox2, sox3, nestin, bmi1, and musashi1 in the ß1^hi population. Cells expressing high levels of ß1 integrin also expressed prominin-1 (CD133), a marker previously used to isolate hNSCs, and selection using integrin ß1^hi cells or prominin-1^hi cells was found to be equally effective at enriching for hNSCs from neurospheres. Therefore, integrin subunits 6 and ß1 are highly expressed by human neural precursors and represent convenient markers for their prospective isolation.

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