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First published online September 14, 2006
Stem Cells Vol. 25 No. 1 January 2007, pp. 174 -180
doi:10.1634/stemcells.2006-0094; www.StemCells.com
© 2007 AlphaMed Press

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EMBRYONIC STEM CELLS

Force Measurements of Human Embryonic Stem Cell-Derived Cardiomyocytes in an In Vitro Transplantation Model

Frank Pillekampa,b, Michael Reppelb, Olga Rubenchykb, Kurt Pfannkucheb, Matthias Matzkiesb, Wilhelm Blochc, Narayanswami Sreerama, Konrad Brockmeiera, Jürgen Heschelerb

aDepartment of Pediatric Cardiology, University of Cologne, Cologne, Germany;
bInstitute of Neurophysiology, University of Cologne, Cologne, Germany;
cDepartment of Molecular and Cellular Sports Medicine, German Sport University, Cologne, Germany

Key Words. Pluripotent stem cells • Myocardial contraction • Cell transplantation • Myocardial infarction • Transplants

Correspondence: Jürgen Hescheler, M.D., Institute of Neurophysiology, University of Cologne, 50931 Cologne, Robert-Koch-Str. 39, Germany. Telephone: ++49-(0)-221-6960; Fax: ++49-(0)-221-3834; e-mail: J.Hescheler{at}uni-koeln.de

Received February 17, 2006; accepted for publication August 28, 2006.
First published online in STEM CELLS EXPRESS   September 14, 2006.



Human embryonic stem cell (hESC)-derived cardiomyocytes have been suggested for cardiac cell replacement therapy. However, there are no data on loaded contractions developed by these cells and the regulation thereof. We developed a novel in vitro transplantation model in which beating cardiomyocytes derived from hESCs (line H1) were isolated and transplanted onto noncontractile, ischemically damaged ventricular slices of murine hearts. After 2–3 days, transplanted cells started to integrate mechanically into the existing matrix, resulting in spontaneous movements of the whole preparation. Preparations showed a length-dependent increase of active tension. In transplanted early beating hESC-derived cardiomyocytes, frequency modulation by field stimulation was limited to a small range around their spontaneous beating rate. Our data demonstrate that this novel in vitro transplantation model is well suited to assess the mechanical properties and functional integration of cells suggested for cardiac replacement strategies.




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[Abstract] [Full Text] [PDF]


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