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TISSUE-SPECIFIC STEM CELLS

Functional Similarities Among Genes Regulated by Oct4 in Human Mesenchymal and Embryonic Stem Cells

^aGraduate School of Biomedical Sciences and
^bDivision of Hematology/Oncology, Department of Medicine, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA

Key Words. OCT4 • Embryonic stem cell • Mesenchymal stem cell • Plasticity Chromatin immunoprecipitation-DNA selection and ligation

Correspondence: Pranela Rameshwar, Ph.D., UMDNJ-New Jersey Medical School, MSB, Room E-579, 185 South Orange Avenue, Newark, New Jersey 07103, USA. Telephone: 973-972-0625; Fax: 973-972-8854; e-mail: rameshwa{at}umdnj.edu

Received on May 8, 2007; accepted for publication on August 21, 2007.

First published online in STEM CELLS EXPRESS  August 30, 2007.


OCT4 is a master transcriptional regulator, which mediates pluripotency in ESCs through inhibition of tissue-specific and promotion of stem cell-specific genes. Suppression of OCT4, along with other regulators of pluripotency, such as SOX2 and NANOG, has been correlated with cell-fate specification and lineage-specific differentiation. Recent reports have shown the expression of OCT4 in adult MSCs but have not ascribed functional homology with ESCs. MSCs are mesoderm-derived cells, primarily resident in adult bone marrow, that undergo lineage-specific differentiation to generate specialized cells such as stroma, fat, bone, and cartilage. We have previously demonstrated the plasticity of MSCs through their ability to generate neuronal cells. Here, we show that OCT4 provides similar regulatory circuitries in human MSCs and ESCs, using chromatin immunoprecipitation-DNA selection and ligation technology and loss-of-function studies. MSCs were found to express the embryonic transcription factors OCT4, NANOG, and SOX2. In addition, OCT4 was found to (a) target similar genes in MSCs and ESCs, (b) promote the expression of MSC-specific genes, and (c) regulate MSC cell cycle progression. The results suggest similar regulatory mechanisms for OCT4 in MSCs and ESCs and have implications regarding MSC plasticity.

Disclosure of potential conflicts of interest is found at the end of this article.

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