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First published online October 12, 2006
Stem Cells Vol. 25 No. 2 February 2007, pp. 481 -489
doi:10.1634/stemcells.2006-0226; www.StemCells.com
© 2007 AlphaMed Press

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EMBRYONIC STEM CELLS: CHARACTERIZATION SERIES

Generation and Characterization of Rabbit Embryonic Stem Cells

Shufen Wanga,b,c, Xianghui Tanga,c, Yuyu Niua,b,c, Hongwei Chena,b,c, Bin Lia,b,c, Tianqing Lia,c, Xiuzhen Zhanga,b,c, Zhixin Hua,b,c, Qi Zhoud, Weizhi Jia,c

aKunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan, China;
bGraduate School, Chinese Academy of Sciences, Beijing, China;
cYunnan Key Laboratory of Animal Reproductive Biology, Kunming Yunnan, China;
dInstitute of Zoology, Chinese Academy of Sciences, Beijing, China

Key Words. Rabbit embryonic stem cells • Parthenogenetic activation • Stem cell marker self-renewal • Signaling pathway

Correspondence: Weizhi Ji, Ph.D., Kunming Primate Research Center and Kunming Institute of Zoology, Chinese Academy of Sciences, 32# Jiaochang Donglu, Kunming Yunnan, 650223, China. Telephone: +86-871-5139413; Fax: +86-871-5139413; e-mail: wji{at}mail.kiz.ac.cn

Received on April 17, 2006; accepted for publication on October 3, 2006.

First published online in STEM CELLS EXPRESS  October 12, 2006.


We described the derivation of four stable pluripotent rabbit embryonic stem cell (ESC) lines, one (RF) from blastocysts fertilized in vivo and cultured in vitro and three (RP01, RP02, and RP03) from parthenogenetic blastocysts. These ESC lines have been cultivated for extended periods (RF >1 year, RP01 >8 months, RP02 >8 months, and RP03 >6 months) in vitro while maintaining expression of pluripotent ESC markers and a normal XY or XX karyotype. The ESCs from all lines expressed alkaline phosphatase, transcription factor Oct-4, stage-specific embryonic antigens (SSEA-1, SSEA-3, and SSEA-4), and the tumor-related antigens (TRA-1-60 and TRA-1-81). Similar to human and mouse ESCs, rabbit ESCs expressed pluripotency (Oct-4, Nanog, SOX2, and UTF-1) and signaling pathway genes (fibroblast growth factor, WNT, and transforming growth factor pathway). Morphologically, rabbit ESCs resembled primate ESCs, whereas their proliferation characteristics were more like those seen in mouse ESCs. Rabbit ESCs were induced to differentiate into many cell types in vitro and formed teratomas with derivatives of the three major germ layers in vivo when injected into severe combined immunodeficient mice. Our results showed that pluripotent, stable ESC lines could be derived from fertilized and parthenote-derived rabbit embryos.




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C. Li, Z. Chen, Z. Liu, J. Huang, W. Zhang, L. Zhou, D. L. Keefe, and L. Liu
Correlation of expression and methylation of imprinted genes with pluripotency of parthenogenetic embryonic stem cells
Hum. Mol. Genet., June 15, 2009; 18(12): 2177 - 2187.
[Abstract] [Full Text] [PDF]


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