Stem Cells http://www.epitomics.com
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online November 16, 2006
Stem Cells Vol. 25 No. 3 March 2007, pp. 562 -570
doi:10.1634/stemcells.2006-0011; www.StemCells.com
© 2007 AlphaMed Press

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2006-0011v1
25/3/562    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamada, M.
Right arrow Articles by Kondo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamada, M.
Right arrow Articles by Kondo, T.

EMBRYONIC STEM CELLS: CHARACTERIZATION SERIES

Electrical Stimulation Modulates Fate Determination of Differentiating Embryonic Stem Cells

Masahisa Yamadaa,b, Kentaro Tanemurac,d, Seiji Okadaf, Akio Iwanamif, Masaya Nakamurag, Hideaki Mizunoe, Michiru Ozawac, Ritsuko Ohyama-Gotoc, Naohito Kitamurab, Masako Kawanoa, Kyoko Tan-Takeuchia, Chiho Ohtsukac, Atsushi Miyawakie, Akihiko Takashimad, Masaharu Ogawaa, Yoshiaki Toyamag, Hideyuki Okanof, Takashi Kondoc

aLaboratory for Cell Culture Development,
bYamada Research Unit, Molecular Neuropathology Group;
cKondo Research Unit, Brain Development Research Group;
dLaboratory for Alzheimer's Disease;
eLaboratory for Cell Function Dynamics, Brain Science Institute, Institute of Physical and Chemical Research, Saitama, Japan;
fDepartment of Physiology and
gDepartment of Orthopaedic Surgery, Keio University School of Medicine, Tokyo, Japan

Key Words. Calcium flux • Tissue regeneration • Neuron • Embryonic stem cells

Correspondence: Takashi Kondo, Ph.D., BSI, RIKEN, Brain Development Research Group, 2-1 Hirosawa Wako Saitama 351-0198 Japan. Telephone: +81 48-467-6729; Fax: +81 48-467-6729; e-mail: tkondo{at}brain.riken.jp

Received January 5, 2006; accepted for publication November 8, 2006.
First published online in STEM CELLS EXPRESS   November 16, 2006.



A clear understanding of cell fate regulation during differentiation is key in successfully using stem cells for therapeutic applications. Here, we report that mild electrical stimulation strongly influences embryonic stem cells to assume a neuronal fate. Although the resulting neuronal cells showed no sign of specific terminal differentiation in culture, they showed potential to differentiate into various types of neurons in vivo, and, in adult mice, contributed to the injured spinal cord as neuronal cells. Induction of calcium ion influx is significant in this differentiation system. This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.epitomics.com
Copyright © 2007 by AlphaMed Press.