HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 2006-0617v1 25/5/1231    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maisel, M. Articles by Storch, A. Search for Related Content PubMed PubMed Citation Articles by Maisel, M. Articles by Storch, A.

TISSUE-SPECIFIC STEM CELLS

Transcription Profiling of Adult and Fetal Human Neuroprogenitors Identifies Divergent Paths to Maintain the Neuroprogenitor Cell State

Departments of ^aNeurology,
^bClinical Genetics, and
^eNeurosurgery, Technical University of Dresden, Dresden, Germany;
^cDepartment of Neurology, University of Leipzig, Leipzig, Germany;
Departments of ^dNeurology,
^fNeurosurgery, and
^gDivision for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopaedics, University of Ulm, Ulm, Germany

Key Words. Gene expression profile • Neural stem cells • Neuroprogenitors • Mesenchymal stem cells • Gene chips

Correspondence: Alexander Storch, M.D., Technical University of Dresden, Department of Neurology, Fetscherstrasse 74, 01307 Dresden, Germany, Telephone: 49-351-458-2532; Fax: 49-351-458-4352; e-mail: alexander.storch{at}neuro.med.tu-dresden.de

Received on September 29, 2006; accepted for publication on January 4, 2007.

First published online in STEM CELLS EXPRESS  January 11, 2007.


Global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs compared with adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern that is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, whereas fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. We present for the first time a detailed transcriptome analysis of human adult NPCs suggesting a relationship between hippocampal NPCs and white matter-derived precursor cells. We further provide a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues.

Disclosure of potential conflicts of interest is found at the end of this article.

This article has been cited by other articles:

				S. Ceruti, G. Villa, T. Genovese, E. Mazzon, R. Longhi, P. Rosa, P. Bramanti, S. Cuzzocrea, and M. P. Abbracchio The P2Y-like receptor GPR17 as a sensor of damage and a new potential target in spinal cord injury Brain, June 15, 2009; (2009) awp147v1. [Abstract] [Full Text] [PDF]