HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 2006-0660v1 25/5/1298    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shin, S. Articles by Chesnut, J. D. Search for Related Content PubMed PubMed Citation Articles by Shin, S. Articles by Chesnut, J. D.

TISSUE-SPECIFIC STEM CELLS

Whole Genome Analysis of Human Neural Stem Cells Derived from Embryonic Stem Cells and Stem and Progenitor Cells Isolated from Fetal Tissue

^aStem Cells and Regenerative Medicine, Invitrogen, Carlsbad, California, USA;
^bBioinformatics Unit, Branch of Research Resources, NIA, NIH, Baltimore, Maryland, USA;
^cThe Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy, Department of Gynecology, Hadassah University Hospital, Jerusalem, Israel;
^dTheradigm Inc., Baltimore, Maryland, USA;
^eThomas Jefferson University, Philadelphia, Pennsylvania, USA;
^fES Cell International Pte Ltd, Helios, Singapore;
^gUniversity of Georgia, Athens, Georgia, USA;
^hUniversity of Rochester, Rochester, New York, USA

Key Words. Large scale genomic analysis • Neural stem cells • Human embryonic stem cells • Adult stem cells • Differentiation Lineage restricted precursor cells

Correspondence: Jon D. Chesnut, Ph.D., 1610 Faraday Ave, Carlsbad, California 92008, USA. Telephone: 760-603-7253; Fax: 760-602-6553 e-mail: jon.chesnut{at}invitrogen.com

Received October 27, 2006; accepted for publication January 19, 2007.
First published online in STEM CELLS EXPRESS   February 1, 2007.



Multipotent neural stem cells (NSC) have been derived from human embryonic stem cells (hESC) as well as isolated from fetal tissues. However, there have been few exclusive markers of NSC identified to date, and the differences between NSC from various sources are poorly understood. Although cells isolated from these two sources share many important characteristics, it is not clear how closely they are related in terms of gene expression. Here, we compare the gene expression profiles of 11 lines of NSC derived from hESC (ES_NSC), four lines of NSC isolated from fetus (F_NSC), and two lines of restricted progenitors in order to characterize these cell populations and identify differences between NSC derived from these two sources. We showed that ES_NSC were clustered together with high transcriptional similarities but were distinguished from F_NSC, oligodendrocyte precursor cells, and astrocyte precursor cells. There were 17 genes expressed in both ES_NSC and F_NSC whose expression was not identified in restricted neural progenitors. Furthermore, the major differences between ES_NSC and F_NSC were mostly observed in genes related to the key neural differentiation pathways. Here, we show that comparison of global gene expression profiles of ES_NSC, F_NSC, and restricted neural progenitor cells makes it possible to identify some of the common characteristics of NSC and differences between similar stem cell populations derived from hESCs or isolated from fetal tissue.

Disclosure of potential conflicts of interest is found at the end of this article.

This article has been cited by other articles:

				G. Esposito, J. Imitola, J. Lu, D. De Filippis, C. Scuderi, V. S. Ganesh, R. Folkerth, J. Hecht, S. Shin, T. Iuvone, et al. Genomic and functional profiling of human Down syndrome neural progenitors implicates S100B and aquaporin 4 in cell injury Hum. Mol. Genet., February 1, 2008; 17(3): 440 - 457. [Abstract] [Full Text] [PDF]