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TISSUE-SPECIFIC STEM CELLS |
aDepartment of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan;
bDepartment of Stem Cell Biology, Cancer Research Institute of Kanazawa University, Kanazawa, Japan;
cPREST, Japan Science and Technology Agency, Saitama, Japan;
dSecond Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan
Key Words. AC133 • Adult bone marrow • Myogenesis • Adipogenesis
Correspondence: Nobuyuki Takakura, M.D., Ph.D., Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan. Telephone: 81-6-6879-8316; Fax: 81-6-6879-8314; e-mail: ntakaku{at}biken.osaka-u.ac.jp; Yoshihiro Yamada, M.D., Ph.D., Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan. Telephone: 81-6-6879-8316; Fax: 81-6-6879-8314; e-mail: yamaday{at}biken.osaka-u.ac.jp
Received on September 19, 2006;
accepted for publication on January 30, 2007.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS February 8, 2007.
Recently, there has been noteworthy progress in the field of cardiac regeneration therapy. We previously reported that brown adipose tissue (BAT) contained cardiac progenitor cells that were relevant to the regeneration of damaged myocardium. In this study, we found that CD133-positive, but not c-Kit- or Sca-1-positive, cells in BAT differentiated into cardiomyocytes (CMs) with a high frequency. Moreover, we found that CD133+ brown adipose tissue-derived cells (BATDCs) effectively induced bone marrow cells (BMCs) into CMs. BMCs are considered to have the greatest potential as a source of CMs, and two sorts of stem cell populations, the MSCs and hematopoietic stem cells (HSCs), have been reported to differentiate into CMs; however, it has not been determined which population is a better source of CMs. Here we show that CD133-positive BATDCs induce BMCs into CMs, not through cell fusion but through bivalent cation-mediated cell-to-cell contact when cocultured. Moreover, BMCs induced by BATDCs are able to act as CM repletion in an in vivo infarction model. Finally, we found that CD45–CD31– CD105+ nonhematopoietic cells, when cocultured with BATDCs, generated more than 20 times the number of CMs compared with lin–c-Kit+ HSCs. Taken together, these data suggest that CD133-positive BATDCs are a useful tool as CM inducers, as well as a source of CMs, and that the nonhematopoietic fraction in bone marrow is also a major source of CMs.
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