HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 2007-0048v1 25/7/1800    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints/Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, M. Articles by Fok, T. F. Search for Related Content PubMed PubMed Citation Articles by Yang, M. Articles by Fok, T. F.

TISSUE-SPECIFIC STEM CELLS

Promoting Effects of Serotonin on Hematopoiesis: Ex Vivo Expansion of Cord Blood CD34⁺ Stem/Progenitor Cells, Proliferation of Bone Marrow Stromal Cells, and Antiapoptosis

^aLi Ka Shing Institute of Health Sciences, Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong, China;
^bDepartment of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong, China;
^cDepartment of Hematology, First Affiliated Hospital, Shantou University Medical College, Shantou, China;
^dLaboratory Animal Services Centre, The Chinese University of Hong Kong, Hong Kong, China

Key Words. Hematopoiesis • CD34⁺ stem cells • Ex vivo expansion • Bone marrow stromal cells • Serotonin • Antiapoptosis

Correspondence: Karen Li, Ph.D., Department of Paediatrics, The Chinese University of Hong Kong, 6^th floor, Clinical Sciences Block, Prince of Wales Hospital, Shatin, New Territories, Hong Kong. Telephone: (852) 2632 2859; Fax: (852) 2636 0020; e-mail: lipang{at}cuhk.edu.hk

Received on January 18, 2007; accepted for publication on March 31, 2007.

First published online in STEM CELLS EXPRESS  April 19, 2007.


Serotonin is a monoamine neurotransmitter that has multiple extraneuronal functions. We previously reported that serotonin exerted mitogenic stimulation on megakaryocytopoiesis mediated by 5-hydroxytryptamine (5-HT)₂ receptors. In this study, we investigated effects of serotonin on ex vivo expansion of human cord blood CD34⁺ cells, bone marrow (BM) stromal cell colony-forming unit-fibroblast (CFU-F) formation, and antiapoptosis of megakaryoblastic M-07e cells. Our results showed that serotonin at 200 nM significantly enhanced the expansion of CD34⁺ cells to early stem/progenitors (CD34⁺ cells, colony-forming unit-mixed [CFU-GEMM]) and multilineage committed progenitors (burst-forming unit/colony-forming unit-erythroid [BFU/CFU-E], colony-forming unit-granulocyte macrophage, colony-forming unit-megakaryocyte, CD61⁺CD41⁺ cells). Serotonin also increased nonobese diabetic/severe combined immunodeficient repopulating cells in the expansion culture in terms of human CD45⁺, CD33⁺, CD14⁺ cells, BFU/CFU-E, and CFU-GEMM engraftment in BM of animals 6 weeks post-transplantation. Serotonin alone or in addition to fibroblast growth factor, platelet-derived growth factor, or vascular endothelial growth factor stimulated BM CFU-F formation. In M-07e cells, serotonin exerted antiapoptotic effects (annexin V, caspase-3, and propidium iodide staining) and reduced mitochondria membrane potential damage. The addition of ketanserin, a competitive antagonist of 5-HT₂ receptor, nullified the antiapoptotic effects of serotonin. Our data suggest the involvement of serotonin in promoting hematopoietic stem cells and the BM microenvironment. Serotonin could be developed for clinical ex vivo expansion of hematopoietic stem cells for transplantation.

Disclosure of potential conflicts of interest is found at the end of this article.

This article has been cited by other articles:

				I. Drobinskaya, T. Linn, T. Saric, R. G. Bretzel, H. Bohlen, J. Hescheler, and E. Kolossov Scalable Selection of Hepatocyte- and Hepatocyte Precursor-Like Cells from Culture of Differentiating Transgenically Modified Murine Embryonic Stem Cells Stem Cells, September 1, 2008; 26(9): 2245 - 2256. [Abstract] [Full Text] [PDF]