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STEM CELL GENETICS AND GENOMICS

Concise Review: Trends in Stem Cell Proteomics

^aDepartment of Stem Cells, Royan Institute, Tehran, Iran;
^bHubrecht Laboratory, Netherlands Institute of Developmental Biology, Utrecht, The Netherlands;
^cDepartment of Biomolecular Mass Spectrometry, Utrecht University, Utrecht, The Netherlands;
^dDepartment of Physiology and Proteomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran

Key Words. Stem cells • Self-renewal • Pluripotent stem cell • Embryonic stem cell biology • Heterogeneity • Proteomics Mass spectrometry • Post-translational modification • Electrophoresis • Quantitative proteomics • Secretomics Two-dimensional electrophoresis

Correspondence: Hossein Baharvand, Ph.D., Department of Stem Cells, Royan Institute, P.O. Box 19395-4644, Tehran, Iran. Telephone: 98-21-22172330; Fax: 98-21-22414532; e-mail: Baharvand50{at}yahoo.com; Ghasem Hosseini Salekdeh, Ph.D., Physiology and Proteomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Karaj, Iran. Telephone: 0098-261-2702893; Fax: 98-261-2704539; e-mail: h_salekdeh{at}abrii.ac.ir

Received on February 8, 2007; accepted for publication on May 4, 2007.

First published online in STEM CELLS EXPRESS  May 10, 2007.


Gene expression analyses of stem cells (SCs) will help to uncover or further define signaling pathways and molecular mechanisms involved in the maintenance of self-renewal, pluripotency, and/or multipotency. In recent years, proteomic approaches have produced a wealth of data identifying proteins and mechanisms involved in SC proliferation and differentiation. Although many proteomics techniques have been developed and improved in peptide and protein separation, as well as mass spectrometry, several important issues, including sample heterogeneity, post-translational modifications, protein-protein interaction, and high-throughput quantification of hydrophobic and low-abundance proteins, still remain to be addressed and require further technical optimization. This review summarizes the methodologies used and the information gathered with proteome analyses of SCs, and it discusses biological and technical challenges for proteomic study of SCs.

Disclosure of potential conflicts of interest is found at the end of this article.

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