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EMBRYONIC STEM CELLS

Rotary Suspension Culture Enhances the Efficiency, Yield, and Homogeneity of Embryoid Body Differentiation

^aThe Wallace H. Coulter Department of Biomedical Engineering and
^bThe Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology/Emory University, Atlanta, Georgia, USA

Key Words. Embryonic stem cells • Embryoid body • Differentiation • Suspension culture

Correspondence: Todd C. McDevitt, Ph.D., 313 Ferst Drive, Suite 2102, Georgia Institute of Technology, Atlanta, Georgia 30332-0535, USA. Telephone: 404-385-6647; Fax: 404-894-4243; e-mail: todd.mcdevitt{at}bme.gatech.edu

Received August 22, 2006; accepted for publication June 8, 2007.
First published online in STEM CELLS EXPRESS   June 21, 2007.



Embryonic stem (ES) cells hold great promise as a robust cell source for cell-based therapies and as a model of early embryonic development. Current experimental methods for differentiation of ES cells via embryoid body (EB) formation are either inherently incapable of larger-scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB agglomeration. This report describes and characterizes a novel method for formation of EBs using rotary orbital motion that simultaneously addresses both concerns. EBs formed under rotary suspension conditions were compared with hanging-drop and static EBs for efficiency of EB formation, cell and EB yield, homogeneity of EB size and shape, and gene expression. A 20-fold enhancement in the number of cells incorporated into primitive EBs in rotary versus static conditions was detected after the first 12 hours, and a fourfold increase in total cell yield was achieved by rotary culture after 7 days. Morphometric analysis of EBs demonstrated formation and maintenance of a more uniform EB population under rotary conditions compared with hanging-drop and static conditions. Quantitative gene expression analysis indicated that rotary EBs differentiated normally, on the basis of expression of ectoderm, endoderm, and mesoderm markers. Increased levels of endoderm gene expression, along with cystic EB formation, indicated by histological examination, suggested that differentiation was accelerated in rotary EBs. Thus, the rotary suspension culture method can produce a highly uniform population of efficiently differentiating EBs in large quantities in a manner that can be easily implemented by basic research laboratories conducting ES cell differentiation studies.

Disclosure of potential conflicts of interest is found at the end of this article.