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TRANSLATIONAL AND CLINICAL RESEARCH

Intra-Bone Marrow Injection of Donor Bone Marrow Cells Suspended in Collagen Gel Retains Injected Cells in Bone Marrow, Resulting in Rapid Hemopoietic Recovery in Mice

^aFirst Department of Pathology,
^bTransplantation Center, and
^cRegeneration Research Center for Intractable Diseases, Kansai Medical University, Osaka, Japan;
^dJapan Immunoresearch Laboratories, Gunma, Japan;
^eDivision of Rheumatology/Allergy and Clinical Immunology, University of California at Davis, Davis, California, USA;
^fSchool of Public Health, Jilin University, Changchun City, China

Key Words. Intra-bone marrow bone marrow transplantation • Collagen gel • Colony-forming units of spleen • Reconstitution • Enhanced green fluorescent protein

Correspondence: Correspondence: Susumu Ikehara, M.D., Ph.D., First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Japan 570-8506. Telephone: 81-6-6992-1001, ext. 2470; Fax: 81-6-6994-8283; e-mail: ikehara{at}takii.kmu.ac.jp

Received on January 12, 2008; accepted for publication on May 24, 2008.

First published online in STEM CELLS EXPRESS  July 3, 2008.

We have recently developed an innovative bone marrow transplantation (BMT) method, intra-bone marrow (IBM)-BMT, in which donor bone marrow cells (BMCs) are injected directly into the recipient bone marrow (BM), resulting in the rapid recovery of donor hemopoiesis and permitting a reduction in radiation doses as a pretreatment for BMT. However, even with this IBM injection, some of the injected BMCs were found to enter into circulation. Therefore, we attempted to modify the method to allow the efficient retention of injected BMCs in the donor BM. The BMCs of enhanced green fluorescent protein transgenic mice (C57BL/6 background) were suspended in collagen gel (CG) or phosphate-buffered saline (PBS), and these cells were then injected into the BM of irradiated C57BL/6 mice. The numbers of retained donor cells in the injected BM, the day 12 colony-forming units of spleen (CFU-S) counts, and the reconstitution of donor cells after IBM-BMT were compared between the CG and PBS groups. The number of transplanted cells detected in the injected BM in the CG group was significantly higher than that in the PBS group. We next carried out CFU-S assays. The spleens of mice in the CG group showed heavier spleen weight and considerably higher CFU-S counts than in the PBS group. Excellent reconstitution of donor hemopoietic cells in the CG group was observed in the long term (>100 days). These results suggest that the IBM injection of BMCs suspended in CG is superior to the injection of BMCs suspended in PBS.

Disclosure of potential conflicts of interest is found at the end of this article.