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TISSUE-SPECIFIC STEM CELLS

In Vivo Osteoprogenitor Potency of Human Stromal Cells from Different Tissues Does Not Correlate with Expression of POU5F1 or Its Pseudogenes

^aThird Department of Internal Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany;
^bDepartment of Experimental Medicine, University La Sapienza and Biomedical Science Park San Raffaele, Rome, Italy;
^cInstitut National de la Santé et de la Recherche Médicale, Equipe ESPRI/EA-3855, Faculty of Medicine, University François Rabelais, Tours, France;
^dInstitut National de la Santé et de la Recherche Médicale U844, Université Montpellier 1, Montpellier, France;
^eDepartment of Gynaecology and Obstetrics, Klinikum rechts der Isar, Munich, Germany;
^fDepartment of Rheumatology and Clinical Immunology, Laboratory For Tissue Engineering, Universitätsklinikum der Charité, Berlin, Germany

Key Words. Mesenchymal stem cells • Stemness • POU5F1 (Oct3/4) • Umbilical vein • Osteoprogenitor

Correspondence: Correspondence: Robert A.J. Oostendorp, Ph.D., Laboratory of Stem Cell Physiology, III Medizinische Klinik und Poliklinik, Klinikum rechts der Isar, Ismaningerstrasse 22, 81675 München, Germany. Telephone: 49-89-4140-6318; Fax: 49-89-4140-6057; e-mail: oostendorp{at}lrz.tum.de

Received on March 25, 2008; accepted for publication on June 19, 2008.

First published online in STEM CELLS EXPRESS  July 10, 2008.

Expression of "stemness" markers is widely used as a predictor of stem cell properties of mesenchymal stem cells (MSC). Here, we show that bone marrow-derived (BM)-MSC show stem cell-like behavior in vivo; that is, they form ossicles with formation of bone, formation of adipocytes, and establishment of the murine hematopoietic microenvironment. Multipotent umbilical vein-derived stromal cells (UVSC), on the other hand, do not form bone, nor do they give rise to adipocytes in vivo. Despite these differences in stem-cell-like behavior, BM-MSC and UVSC express the two transcripts variants of POU5F1 at a similar level. Also, we found that in BM-MSC and UVSC, POU5F1 is detectable. However, more than 89% of the POU5F1 transcripts correspond to the POU5F1P1, -P3, or -P4 pseudogene. Despite low-level expression of POU5F1, we were unable to precipitate POU5F1 protein in either BM-MSC or UVSC. These results demonstrate that MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes.

Disclosure of potential conflicts of interest is found at the end of this article.

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