International Journal of Cell Cloning, Vol 3, 294-303, Copyright © 1985 by AlphaMed Press
CSF producing gall bladder cancer: case report and characteristics of the CSF produced by tumor cells
M Takahashi, M Fujiwara, K Kishi, C Sakai, M Sanada, Y Moriyama and A Shibata
Due to an increase in mature neutrophils, a 72-year-old female with gall
bladder cancer showed leukocytosis of up to 1.32 X 10(11)/l; hypercalcemia
was up to 7.7 mEq/l in her terminal stage. Leukocyte counts and calcium
values increased as the tumor progressed. There was no sign of infection or
bone marrow metastasis. Cultured cells from the tumor tissue produced high
colony-stimulating factor (CSF) activity into the supernatant. The tumor
cell-conditioned medium stimulated exclusively granulocytic colonies. The
study of this patient shows that leukocytosis was caused by the CSF
produced by tumor cells. Approximately 90% of CSF activity was lost by heat
treatment at 60 degrees C for 30 min. The CSF was stable over a pH range of
3-11 and was inactivated by treatment with proteolytic enzymes, but was not
affected by treatment with DNase or neuraminidase. Molecular weight of the
CSF, demonstrated by fractionation using Sephacryl S-200, was approximately
27,000 to 30,000.
