International Journal of Cell Cloning, Vol 4, 115-125, Copyright © 1986 by AlphaMed Press
1 alpha, 25-Dihydroxyvitamin D3 augments clonal growth of macrophages from rat bone marrow progenitor cells and modulates their function
E Shezen and R Goldman
1 alpha, 25-Dihydroxyvitamin D3 (1,25(OH)2D3) was shown to enhance
(approximately 2 fold) the colony-stimulating factor-dependent clonal
growth of macrophage colonies and clusters from rat bone marrow progenitor
cells. The proliferative capacity of macrophage progenitors in liquid
cultures was likewise augmented (2-3 fold). Mononuclear phagocytes
(macrophages, for simplicity) developing in the presence of 1,25(OH)2D3
showed a reduced capacity of migration. 1,25(OH)2D3 administered at bone
marrow culture initiation led to augmentation of the phagocytic capability
of macrophages in four-day cultures and to its suppression in macrophages
in seven-day cultures. The observed patterns of modulation of
differentiation and function by 1,25(OH)2D3 differ from the patterns we
found for mouse bone marrow cells. The results suggest that the
differential response to hormones observed in different species may include
responses to 1,25(OH)2D3.
