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International Journal of Cell Cloning, Vol 4, 192-202, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Effect of 5-azacytidine on the differentiation of human leukemia K-562 cells

R Sasaki and Y Miura

The treatment of K-562 cells with 10(-5) M to 10(-7) M 5-azacytidine induced a marked increase in benzidine-positive cells. Similarly, the exposure of K-562 cells to 2 X 10(-3) M butyric acid or 5 X 10(-7) M 1- beta-arabinofuranosylcytosine or 1 X 10(-3) M hydroxyurea induced an erythroid differentiation of K-562 cells. The activity of DNA- methyltransferase and the level of methylcytosine in newly synthesized DNA were significantly decreased when the cells were treated with 5- azacytidine or butyric acid, while 1-beta-arabinofuranosylcytosine or hydroxyurea had no inhibitory effect on DNA-methylation of K-562 cells. These results suggest that the inhibition of DNA-methylation is not necessarily a specific phenomenon for erythroid differentiation of K- 562 cells.




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