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International Journal of Cell Cloning, Vol 4, 279-284, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Proliferative characteristics of progeny derived from normal human hemopoietic progenitor cells

PE Crossen, JM Godwin and MP Bodger

Cell cycle times of cells derived from human hemopoietic progenitor cells were measured directly by using 5-bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. Umbilical cord cells were cultured in the mixed-colony assay for 96 h prior to the addition of BrdU and then harvested at 6-12 h intervals. Individual cell cycle times ranging from 8.5-23.4 h were observed while population mean cell cycle times ranged from 17.5-24 h. These results show that some early progeny of hemopoietic progenitor cells have short cell cycle times and that there is considerable heterogeneity within the proliferating cell population.




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