Stem Cells http://www.stemcellsportal.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Keng, P. C.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Keng, P. C.

International Journal of Cell Cloning, Vol 4, 295-311, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Use of flow cytometry in the measurement of cell mitotic cycle

PC Keng

Variations in many cellular characteristics during the cell cycle can be analyzed simply and directly by flow cytometry. Using multiparameter analysis of DNA content, RNA content, cell size and 5-bromodeoxyuridine (BrdUrd) incorporation, it is now possible to define cells' positions in the cell cycle with a precision previously unimaginable. It is also possible, by using the sorting function of the flow cytometer, to separate populations in different phases of the cell cycle for biological and biochemical studies. This review describes the technical aspects of flow cytometric instrumentation, DNA staining procedures, and the cytometric applications of both in cell cycle analysis including some of the more innovative, new approaches with antibody against BrdUrd.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 1986 by AlphaMed Press.