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International Journal of Cell Cloning, Vol 4, 424-431, Copyright © 1986 by AlphaMed Press

ORIGINAL ARTICLES

Cloning of early erythroid and mixed myeloid/erythroid human bone marrow progenitor cells: comparison of different sources of burst- promoting activity (BPA)

HH Gerhartz and W Wilmanns

The ability of conditioned media from the 5637 cell line and human placenta (HPCM) to stimulate the in vitro growth of human early erythroid and mixed myeloid/erythroid clones was tested and compared to phytohemagglutinin-stimulated leukocyte supernatants (PHA-LCM). Both 5637 supernatant and PHA-LCM were equally effective with a linear dose- response relationship. HPCM at various concentrations did not exhibit burst-promoting activity (BPA). Thus, "pluripoietin"-containing media provide a large-scale source of BPA similar in its biological activity to standard sources used for studying human hematopoiesis in vitro.




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