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International Journal of Cell Cloning, Vol 5, 315-321, Copyright © 1987 by AlphaMed Press

ORIGINAL ARTICLES

Growth of murine bone marrow under various oxygen conditions in media buffered with HEPES

MJ Allalunis-Turner, CJ Kock and JD Chapman

Increased plating efficiencies were observed for marrow granulocyte- macrophage precursor cells (CFU-gm) cultured in an experimental system at reduced gas-phase oxygen concentrations. However, only a small component of this increase was attributable to decreased oxygen. The changed media buffering system was the more dominant factor contributing to increased plating efficiency, suggesting that other factors in addition to a reduction in oxygen may influence clonogenicity.




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Copyright © 1987 by AlphaMed Press.