International Journal of Cell Cloning, Vol 7, 30-36, Copyright © 1989 by AlphaMed Press
Effects of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on single CD34-positive hemopoietic progenitors from human bone marrow
H Mayani, P Baines, A Jones, T Hoy and A Jacobs
Department of Haematology, University Hospital of Wales, Heath Park, Cardiff, UK.
To determine the extent accessory cells mediate the effects of recombinant
human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on human
hemopoietic progenitors in vitro, we added this hemopoietin to liquid
cultures of single CD34-positive marrow cells. These were selected on a
fluorescence-activated cell sorter using the HPCA-1 (My10) antibody.
Myeloid, erythroid and a few mixed clones developed in 13% of wells in the
apparent absence of accessory cells at the beginning of culture. Although
accessory cells were generated quickly from the myeloid progenitors and
could have mediated the action of rhGM-CSF, this was not the case in the
majority of the erythroid clones in which no other cell types were
recorded. We conclude that rhGM-CSF can act directly on a subset of
erythroid progenitors and probably induces a substantial number of myeloid
clones directly.
