International Journal of Cell Cloning, Vol 8, 39-53, Copyright © 1990 by AlphaMed Press
Specific binding of 125I-rErythropoietin to Friend polycythemia virus- transformed erythroleukemia cells purified by centrifugal elutriation
PN Correa, V Bard and AA Axelrad
Department of Anatomy, University of Toronto, Canada.
We have used countercurrent centrifugal elutriation (CCE) to determine the
distribution of cells with respect to cell volume and buoyant density for
an erythroleukemia cell line (JG6) transformed by the polycythemia strain
of Friend virus (FV-P), and to determine the effect of inducing the cells
to differentiate with dimethylsulfoxide (DMSO) on this distribution. CCE
made it possible to obtain suspensions of modal JG6 populations virtually
free of dead cells and uniform with respect to volume and buoyant density.
These modal populations were assayed for specific binding of erythropoietin
(Epo). Between 500 and 550 Epo receptors per cell were detected. These
belonged to a single class having a dissociation constant of 0.36 nM. DMSO
induction of differentiation of the JG6 cells had no effect on the number
of Epo receptors expressed.
