International Journal of Cell Cloning, Vol 8, 123-129, Copyright © 1990 by AlphaMed Press
Stimulation of myeloid colony growth from peripheral blood by medium with an initially low osmolality
GB Tennant
Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
The clonal growth of myeloid colonies from peripheral blood was maximal
when cultures were established with an initial osmolality of 220 mosmol/kg
which increased during incubation as a result of partial drying. When
osmolality was stabilized by secondary humidification, the optimum
osmolality was 270 mosmol/kg, but growth was always two- to fivefold less
than similar cultures established at low osmolality and incubated on an
open shelf. Cultures established at 270 mosmol/kg or above were
statistically similar whether or not drying was eliminated. Maximum
colonies were apparent after 14 days incubation under both conditions;
addition of conditioned medium did not alter the pattern of growth. The
greater sensitivity of cultures established at 220 mosmol/kg is
advantageous when assaying circulating progenitors in pathological
conditions where a low number of granulocyte/macrophage colony-forming
units is common.
