International Journal of Cell Cloning, Vol 8, 196-208, Copyright © 1990 by AlphaMed Press
Comparative distribution of marrow CFU-e and CFU-gm progenitors in different anatomic sites in the dog
A Deldar, CE Stevens and PJ Beineke
Toxicology Division, Lilly Research Laboratories, Eli Lilly and Co., Greenfield, Indiana 46140.
The interpretation of marrow cloning activity, particularly in serial
cultures, is greatly influenced by the reproducibility of the collected
marrow samples. In order to establish whether bone marrow cloning
activities and precision of the cloning assays are influenced by the site
of bone marrow collection in the dog, we studied the incidence of marrow
erythroid (CFU-e) and granulocyte-macrophage (CFU-gm) progenitor cells in
the iliac crest, sternum, vertebrae, femur, and humerus, using microplasma
clot and soft agar culture systems. Marrow samples obtained from the femur
and humerus revealed consistently higher cell concentrations than those
from the iliac crest, vertebrae, or sternum. Those aspirated from the
sternum and vertebrae had lower cell concentrations and were less
reproducible. Statistical analysis revealed no significant differences in
the incidence of marrow CFU-e and CFU-gm progenitor cells between the
femur, humerus, iliac crest or vertebrae. With multiple sampling, the
marrow cloning efficiency was consistent and reproducible within the
individual dogs. We conclude that the distribution of CFU-e and CFU-gm is
comparable throughout the active marrow in the dog and that these sites may
be used interchangeably for multiple quantitative analysis of marrow
hematopoietic progenitor cells.
