International Journal of Cell Cloning, Vol 8, 377-384, Copyright © 1990 by AlphaMed Press
The effect of carbamylcholine on CFU-s differentiation
XT Hu, YH Xu and YJ Zhou
Research Laboratory of Blood Physiology, Hunan Medical University, Changsha, People's Republic of China.
The proportion of spleen colony-forming units (CFU-s) killed by hydroxyurea
was greatly increased after bone marrow cells (BMCs) from LACA mice were
exposed to carbamylcholine (Cach; 1 X 10(-13) to 1 X 10(- 9) in vitro and
there was a marked change in the proportion of spleen colony types.
Following treatment with Cach, granulocytic and mixed erythroid-type
colonies increased from 20 to 26.3% and 16.1 to 29.6% in 9-day colonies and
from 8.3 to 28.2% and 21.7 to 39.4% in 13-day colonies, respectively.
Single cell suspensions of spleen colonies were made for
granulocyte-macrophage progenitor (CFU-gm) and late erythroid progenitor
(CFU-e) assays. The number of CFU-gm from Cach-treated BMC was about twice
that from control BMC for both day 9 and day 13 groups; the number of CFU-e
decreased relatively. The results suggest that cholinergic receptors on
CFU-s may increase the tendency to differentiate into the
granulocytic/monocytic line.
