Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilks, D. P.
Right arrow Articles by West, C. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Wilks, D. P.
Right arrow Articles by West, C. M.

International Journal of Cell Cloning, Vol 9, 559-569, Copyright © 1991 by AlphaMed Press

ORIGINAL ARTICLES

A serum-free medium for the Courtenay-Mills soft agar assay

DP Wilks and CM West
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, United Kingdom.

A serum-free medium has been developed to support the clonogenic growth in soft agar of human cervical carcinoma cell lines xenografted into nude mice. Using the Courtenay-Mills assay, colony-forming efficiencies and colony sizes equivalent to those obtained using Ham's F12 plus 15% fetal calf serum can be obtained. Validation of the assay using the developed medium was obtained through establishing linearity between the number of cells plated and colonies formed, and by producing radiation survival curves.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.stemcellsportal.com/
Copyright © 1991 by AlphaMed Press.