| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original Article |
1 Taipei Medical University
2 Taipei Medical University and Wan-Fang Hospital
* To whom correspondence should be addressed. E-mail: danieltb_shih{at}yahoo.com.tw.
 |
Abstract |
|---|
Recent studies have shown that adult tissues contain stem/progenitor cells capable of not only generating mature cells of their tissue of origin, but also trans-differentiating themselves into other tissue cells. Murine skin derived precursor cells (mSKPs), for example, have been described as unique, non-mesenchymal-like stem cells capable of mesodermal and ectodermal neurogenic differentiation [17]. Human derived skin precursors are less well characterized.
In this study, the isolation, and characterization of adherent, mesenchymal stem cell-like cells from human scalp tissue (hSCPs) was described. hSCPs initially isolated by medium selection (ms-hSCP) and by single cell (c-hSCP) were cultured with consistent growth rate in medium containing EGF, and FGF-
. Cultured ms-hSCP and c-hSCP displayed undistinquishable stable phenotype and continuously replicated in cell culture. Both hSCPs expressed surface antigen profile (CDw90, SH2, SH4, CD105, CD166, CD44, CD49d-e and HLA class I) similar to that of BM-MSCs. The growth kinetics, surface epitopes, and differentiation potential of c-hSCP cells were characterized and compared to bone marrow mesenchymal stem cells (BM-MSCs). In addition to differentiation along the osteogenic, chondrogenic, and adipogenic lineages, hSCPs can effectively differentiate into neuronal precursors evident by neurogenic gene expression of GFAP, NCAM, NF-M and MAP2 transcripts. Therefore, hSCPs may potentially be useful as a better alternative MSCs for the neural repairing, in addition to their other mesenchymal regenerative capacity. Our study suggests that hSCPs may provide an alternative adult stem cell resource that may useful for regenerative tissue repair and auto-transplantations.
This article has been cited by other articles:
|
|
 |
|
  E. F. Wolff, A. B. Wolff, Hongling Du, and H. S. Taylor Demonstration of Multipotent Stem Cells in the Adult Human Endometrium by In Vitro Chondrogenesis Reproductive Sciences, September 1, 2007; 14(6): 524 - 533. [Abstract] [PDF]
|
|
|
|
 |
|
 C. E. Wong, C. Paratore, M. T. Dours-Zimmermann, A. Rochat, T. Pietri, U. Suter, D. R. Zimmermann, S. Dufour, J. P. Thiery, D. Meijer, et al. Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin J. Cell Biol., December 18, 2006; 175(6): 1005 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Meindl, U. Schmidt, C. Vaculik, and A. Elbe-Burger Characterization, isolation, and differentiation of murine skin cells expressing hematopoietic stem cell markers J. Leukoc. Biol., October 1, 2006; 80(4): 816 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yu, D. Fang, S. M. Kumar, L. Li, T. K. Nguyen, G. Acs, M. Herlyn, and X. Xu Isolation of a Novel Population of Multipotent Adult Stem Cells from Human Hair Follicles Am. J. Pathol., June 1, 2006; 168(6): 1879 - 1888. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
