Disparate Mesenchyme-Lineage Tendencies in Mesenchymal Stem Cells from Human Bone Marrow and Umbilical Cord Blood

¹ Food Industry Research and Development Institute, Hsinchu, Taiwan
² Taipei Medical University, Taipei, Taiwan
³ National Chiao Tung University, Hsinchu, Taiwan

^* To whom correspondence should be addressed. E-mail: hsm{at}firdi.org.tw.

	Abstract

Bone marrow and umbilical cord blood are reported to be the main sources of mesenchymal stem cells (MSCs), which have been proposed for many clinical applications. This study evaluated and quantitated the differentiation potential of bone marrow-derived MSCs (bmMSCs) and cord blood-derived MSCs (cbMSCs) by in vitro induction. Results indicated that cbMSCs had a significantly stronger osteogenic potential but less capacity in adipogenic differentiation than bmMSCs. Leptin, an important regulator of mesenchymal differentiation, has a significant stronger effect to promote osteogenesis and inhibit adipogenesis in bmMSCs than cbMSCs. Moreover, Cbfa1 mRNA expression in bmMSCs and cbMSCs was affected at different degree by leptin during osteogenesis. In contrast, leptin reduced PPAR2 mRNA expression at same level during adipogenesis in both types of MSCs. These results demonstrate the disparate capacity of MSCs from bone marrow and cord blood and suggest that they be used differently in experimental and therapeutic studies. In addition, the disparate differentiation tendency of MSCs from different sources should be considered in further applications.

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