Submitted on February 17, 2005
Accepted on May 5, 2005
Expression of Cytochrome P450 Genes in CD34+ Hematopoietic Stem and Progenitor Cells
Pavel Sou
ek 1*,
Pavel Anzenbacher 2,
Ivana Skoumalová 3,
Michal Dvo
ák 4
1 Biotransformations Group, National Institute of Public Health, Center of Occupational Diseases, 
robárova 48, Praha 10, Czech Republic
2 Institute of Pharmacology, Faculty of Medicine, Palacky University at Olomouc, Olomouc, Czech Republic
3 Hematooncological Department, Faculty Hospital Olomouc, Olomouc, Czech Republic
4 Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 37 Prague, Czech Republic
* To whom correspondence should be addressed. E-mail: psoucek{at}szu.cz.
 |
Abstract |
|---|
Expression of major cytochrome P450 forms (P450) was followed in preparation of purified hematopoietic CD34+ stem and progenitor cells. Levels of transcripts as well as mature proteins were traced by quantitative RealTime-PCR and by Northern and Western blotting. P450 1B1 and P450 2E1 proteins and respective mRNAs were found in all cases. On the other hand, no expression P450 3A4, 3A7 and P450 2C9 was found. The results show that various P450 enzymes start to be expressed at various degrees of cell differentiation. Both P450 forms found are known to be connected with cancer cells and with activation of procarcinogens (P450 1B1, polycyclic aromatic hydrocarbons; P450 2E1, nitrosamines and solvents). Hence, already cells at the early stage of differentiation may be influenced by interaction with xenobiotics. This fact should also be taken into consideration when hematopoietic cell transplant therapy is applied.
Key Words.
CD34+, cytochrome P450, expression, progenitor cells, Real-Time PCR
