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Original Article |
1 Research and Development, Finnish Red Cross Blood Service, Helsinki, Finland
2 Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA, USA
3 Institute of Signal Processing, Tampere University of Technology, Tampere, Finland
4 Research and Development, Finnish Red Cross Blood Service, Helsinki, Finland; Department of Tissue Typing, Finnish Red Cross Blood Service, Helsinki, Finland
* To whom correspondence should be addressed. E-mail: jukka.partanen{at}bts.redcross.fi.
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Abstract |
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Human cord blood (CB)-derived CD133+ cells carry characteristics of primitive hematopoietic cells and proffer an alternative for CD34+ cells in hematopoietic stem cell (HSC) transplantation. To characterize the CD133+ cell population on genetic level, a global expression analysis of CD133+ cells was performed using oligonucleotide microarrays. CD133+ cells were purified from 4 fresh CB units by immunomagnetic selection. All 4 CD133+ samples showed significant similarity in their gene expression pattern, whereas they differed clearly from the CD133- control samples. In all, 690 transcripts were differentially expressed between CD133+ and CD133- cells. Of these, 393 were increased and 297 were decreased in CD133+ cells. The highest overexpression was noted in genes associated with metabolism, cellular physiological processes, cell communication and development. A set of 257 transcripts expressed solely in the CD133+ cell population was identified. Colony-forming unit (CFU) assay was used to detect the clonal progeny of precursors present in the studied cell populations. The results demonstrate that CD133+ cells express primitive markers and they possess clonogenic progenitor capacity. This study provides a gene expression profile for human CD133+ cells. It presents a set of genes that may be utilized to unravel the properties of the CD133+ cell population, assumed to be highly enriched in HSCs.
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