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First published online October 20, 2005
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2005-0194v1
24/3/547    most recent
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Submitted on April 27, 2005
Accepted on October 12, 2005

Original Article

Cell-cycle features of primate embryonic stem cells

Anne-Catherine Fluckiger 1, Guillaume Marcy 1, Mélanie Marchand 1, Didier Nègre 2, François-Loïc Cosset 2, Shoukhrat Mitalipov 3, Don Wolf 3, Pierre Savatier 1*, Colette Dehay 1

1 INSERM U371 "Cerveau et Vision", Bron, France
2 INSERM U412, Lyon, France
3 Oregon National Primate Research Center, Oregan Health & Science University, Beaverton, Oregon

* To whom correspondence should be addressed. E-mail: savatier{at}lyon.inserm.fr.


  Abstract

Using flow cytometry measurements combined with quantitative analysis of cell-cycle kinetics, we show that rhesus monkey ES cells are characterised by an extremely rapid transit through the G1 phase which accounts for 15% of the total cell-cycle duration. Monkey ES cells exhibit a non phasic expression of cyclin E which is detected during all phases of the cell-cycle and do not growth-arrest in G1 following {gamma}-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of Mitogen Activated Protein Kinase Kinase (MEK) did not result in any alteration in the cell-cycle distribution, indicating that ES cell growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ES cells, like their murine counterparts, exhibit unusual cell-cycle features in which cell-cycle control mechanisms operating during the G1 phase are reduced or absent.

Key Words. Rhesus ES cells, cell-cycle, stemness




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