| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original Article |
1 Kansai Medical University, Osaka, Japan
2 Kyoto Institute of Technology, Kyoto, Japan
3 School of Public Health, Jilin University, Changchun, China
4 University of California at Davis, Davis, California
* To whom correspondence should be addressed. E-mail: ikehara{at}takii.kmu.ac.jp.
 |
Abstract |
|---|
Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs have some features (surface molecules and cytokine production, etc.) common to so-called traditional bone marrow (BM) stromal cells, which have the capacity to support hemopoiesis. In the present study, we isolated murine MSCs (mMSCs) from the fetal BM using an anti-PA6 mAb that is specific for bone marrow stromal cells. The mMSCs, called FMS/PA6-P cells, are adherent, fibroblastic, extensively expanded and have the ability to differentiate not only into osteoblasts and adipocytes but also into vascular endothelial cells. The FMS/PA6-P cells produce a broad spectrum of cytokines and growth factors closely related to hemopoiesis, and show good hemopoiesis-supporting capacity both in vivo and in vitro, suggesting that they are a component of the HSC-niche in vivo. Interestingly, although the FMS/PA6-P cells express a high level of the PA6 molecule, which is reactive with anti-PA6 mAb, they gradually lose their ability to express this molecule during the course of differentiation into osteoblasts and adipocytes, indicating that the PA6 molecule might serve as a novel marker of mMSCs.
Key Words. mesenchymal stem cells, hematopoietic stem cells, bone marrow cells, PA6, mouse
This article has been cited by other articles:
|
|
 |
|
  Y.-W. Kim, B.-K. Koo, H.-W. Jeong, M.-J. Yoon, R. Song, J. Shin, D.-C. Jeong, S.-H. Kim, and Y.-Y. Kong Defective Notch activation in microenvironment leads to myeloproliferative disease Blood, December 1, 2008; 112(12): 4628 - 4638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Geske, X. Zhang, K. K. Patel, D. M. Ornitz, and T. S. Stappenbeck Fgf9 signaling regulates small intestinal elongation and mesenchymal development Development, September 1, 2008; 135(17): 2959 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ma, M. Shi, J. Li, B. Chen, H. Wang, B. Li, J. Hu, Y. Cao, B. Fang, and R. C. Zhao Senescence-unrelated impediment of osteogenesis from Flk1+ bone marrow mesenchymal stem cells induced by total body irradiation and its contribution to long-term bone and hematopoietic injury Haematologica, July 1, 2007; 92(7): 889 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Li, H. Hisha, R. Yasumizu, T.-X. Fan, G.-X. Yang, Q. Li, Y.-Z. Cui, X.-L. Wang, C.-Y. Song, S. Okazaki, et al. Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes Stem Cells, May 1, 2007; 25(5): 1186 - 1194. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| STEM CELLS | THE ONCOLOGIST | CME | ALPHAMED PRESS JOURNALS |
