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Commentary |
1 Burnham Institute for Medical Research, La Jolla, California
2 Johns Hopkins University, Baltimore, Maryland
* To whom correspondence should be addressed. E-mail: mahendrasrao{at}verizon.net.
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Abstract |
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As of August 2005, 22 human ES cell lines listed on the NIH Stem Cell Registry were being distributed to investigators. At a meeting in June, 2005 of NIH-supported human ES cell researchers, we proposed that a set of shared standards should be available in order to characterize the cells unambiguously in multiple laboratories. Here we elaborate such a plan to identify a set of standard methods and to initiate collaborative efforts to validate the standards. The standard assays we propose should be comprehensive enough to assure that human ES cell banks can provide a consistent and reliable product for NIH researchers, and inexpensive enough that individual laboratories can afford to use at least some of the methods routinely in their laboratories. We expect that as data are accumulated and standards evolve, a core set of tests will become the norm for routine assessment of hESC cultures and these tests will lay the groundwork for clinical applications of these cells.
Key Words. hESC, karyotype, characterization, mitochondrial sequencing, methylation
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