Stem Cells http://www.peprotech.com/
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online June 22, 2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-0625v1
24/10/2262    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tjabringa, G.S.
Right arrow Articles by Klein-Nulend, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tjabringa, G.S.
Right arrow Articles by Klein-Nulend, J.
Submitted on December 13, 2005
Accepted on June 15, 2006

Tissue-Specific Stem Cells

POLYAMINES MODULATE NITRIC OXIDE PRODUCTION AND COX-2 GENE EXPRESSION IN RESPONSE TO MECHANICAL LOADING IN HUMAN ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS

G.S. Tjabringa 1, P.S. Vezeridis 1, B. Zandieh-Doulabi 1, M.N. Helder 2, P.I.J.M. Wuisman 3, J. Klein-Nulend 1*

1 Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam (ACTA), Universiteit van Amsterdam (UvA), and Vrije Universiteit, Amsterdam, The Netherlands
2 Department of Oral Cell Biology, Academic Center of Dentistry Amsterdam (ACTA), Universiteit van Amsterdam (UvA), and Vrije Universiteit, Amsterdam, The Netherlands; Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands
3 Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands

* To whom correspondence should be addressed. E-mail: j.kleinnulend{at}vumc.nl.


  Abstract

For bone tissue engineering, it is important that mesenchymal stem cells (MSCs) display a bone cell-like response to mechanical loading. We have shown earlier that this response includes increased nitric oxide (NO) production and cyclooxygenase-2 (COX-2) gene expression, both of which are intimately involved in mechanical adaptation of bone. COX-2 gene expression is likely regulated by polyamines, which are organic cations implicated in cell proliferation and differentiation. This has led to the hypothesis that polyamines may play a role in the response of adipose tissue-derived MSCs (ATMSCs) to mechanical loading. The aim of this study was to investigate whether genes involved in polyamine metabolism are regulated by mechanical loading and to study whether polyamines modulate mechanical loading-induced NO production and COX-2 gene expression in human ATMSCs.

Human AT-MSCs displayed a bone cell-like response to mechanical loading applied by pulsating fluid flow (PFF), as demonstrated by increased NO production and increased gene expression of COX-2 . Furthermore, PFF increased gene expression of spermidine/spermine N(1)- acetyltransferase (SSAT), which is involved in polyamine catabolism, suggesting that mechanical loading modulates polyamine levels. Finally, the polyamine spermine was shown to inhibit both PFFinduced NO production and COX-2 gene expression, suggesting that polyamines modulate the response of human AT-MSCs to mechanical loading.

In conclusion, this is the first study implicating polyamines in the response of human AT-MSCs to mechanical loading, creating opportunities for

Key Words. Polyamines, adipose tissue-derived mesenchymal stem cells, mechanical loading, fluid shear stress, fluid flow, nitric oxide, spermidine/spermine N(1)-acetyltransferase, cyclooxygenase-2, bone cells




This article has been cited by other articles:


Home page
 Stem CellsHome page
A. Schaffler and C. Buchler
Concise Review: Adipose Tissue-Derived Stromal Cells--Basic and Clinical Implications for Novel Cell-Based Therapies
Stem Cells, April 1, 2007; 25(4): 818 - 827.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2006 by AlphaMed Press.