Stem Cells
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First published online April 27, 2006
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2005-0648v1
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Submitted on December 23, 2005
Accepted on April 17, 2006

Embryonic Stem Cells

Differentiation of Human Embryonic Stem Cells Into bipotent Mesenchymal Stem Cells

Emmanuel N. Olivier 1, Anne C. Rybicki 2, Eric E. Bouhassira 1*

1 Einstein Center for Human Embryonic Stem Cell Research, Department of Medicine, Division of Hematology and Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York
2 Department of Medicine, Division of Hematology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York

* To whom correspondence should be addressed. E-mail: bouhassi{at}aecom.yu.edu.


  Abstract

Mesenchymal stem cells (MSC) are multipotent progenitors that can be found in many connective tissues including fat, bone, cartilage and muscle. We report here a method to reproducibly differentiate human embryonic stem cells (hESCs) into MSCs that does not require the use of any feeder layer. The cells obtained with this procedure are morphologically similar to bone marrow MSCs, are contact-inhibited, can be grown in culture for about 20-25 passages, have a immuno-phenotype similar to bone marrow MSCs (negative for CD34, CD45 and positive for CD13, CD44, CD71, CD73, CD105, CD166, HLA ABC and SSEA4), can differentiate into osteocytes and adipocytes and can be used as feeder cells to support the growth of undifferentiated hESCs cells. The ability to produce MSCs from hESCs should prove useful to produce large amounts of genetically identical and genetically modifiable MSCs that can be used to study the biology of MSCs and for therapeutic applications.




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