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Embryonic Stem Cells |
1 Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, Oregon
2 Stem Cell Program, Genomics Research Center, and Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
3 Cytogenetics Laboratory, Wisconsin State Laboratory of Hygiene, Madison, Wisconsin; Cell Line Genetics, Research Park, Madison, Wisconsin
4 Cytogenetics Laboratory, Wisconsin State Laboratory of Hygiene, Madison, Wisconsin
* To whom correspondence should be addressed. E-mail: mitalipo{at}ohsu.edu.
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Abstract |
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Embryonic stem cells (ESC) are important as research subjects since the mechanisms underlying cellular differentiation, expansion and self renewal can be studied along with differentiated tissue development and regeneration in vitro. Furthermore, human ESCs hold promise for cell and tissue replacement approaches to treating human diseases. The rhesus monkey is a clinically relevant primate model that will likely be required to bring these clinical applications to fruition. Monkey ESCs share a number of properties with human ESCs and their derivation and use is not impacted by bioethical concerns. Here, we summarize our experience in the establishment of eighteen ESC lines from rhesus monkey preimplantation embryos generated by the application of the assisted reproductive technologies. The newly derived monkey ESC lines were maintained in vitro without losing their chromosomal integrity and expressed markers previously reported present in human and monkey ESCs. We also describe initial efforts to compare the pluripotency of ESC lines by expression profiling, chimeric embryo formation and in vitro directed differentiation into endodermal, mesodermal and ectodermal lineages.
Key Words. embryonic stem cells, chimera, karyotype, rhesus monkey, transcriptome
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