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Tissue-Specific Stem Cells |
1 The Ira B. Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology; M.D./Ph.D. Program, Graduate School of Biomedical Sciences, University of Medicine and Denistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey; The Joint Program in Toxicology, Rutgers University, Piscataway, New Jersey
2 The Ira B. Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology; M.D./Ph.D. Program, Graduate School of Biomedical Sciences, University of Medicine and Denistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey
3 The Ira B. Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Denistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey
* To whom correspondence should be addressed. E-mail: coynetm{at}umdnj.edu.
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Abstract |
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The remarkable plasticity of marrow stromal cells (MSCs) after transplantation to models of neurological disease and injury has been described. In this report, we investigated the plasticity and long-term survival of MSCs transplanted into the normal brain. MSCs were isolated from GFP transgenic rats and double-labeled with BrdU and bis benzamide prior to transplantation into the adult hippocampus or striatum. Surgery elicited an immediate inflammatory response. MSC grafts were massively infiltrated by ED1-positive microglia/macrophages and surrounded by a marked astrogliosis. By 14 days, graft volume had retracted and GFP immunoreactivity was absent, indicating complete donor rejection. Consequently, MSCs did not exhibit plasticity formerly identified in other studies. However, BrdU and bis benzamide-labeled cells were detected up to 12 weeks. Control transplants of non-viable MSCs demonstrated the transfer of donor labels to host cells. Unexpectedly, BrdU-labeling was colocalized to host phagocytes, astrocytes, and neurons in both regions. Our results indicate that MSCs transplanted to the intact adult brain are rejected by an inflammatory response. Moreover, use of the traditional cell labels BrdU and bis benzamide may provide a misleading index of donor survival and differentiation after transplantation.
Key Words. Mesenchymal Stem Cells, Bone Marrow Stromal Cells, Cell Transplantation, Mesenchymal Stem Cell Transplantation, Graft Rejection
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