Constitutive Gene Expression Predisposes Morphogen-Mediated Cell Fate Responses of NT2/D1 and 27X-1 Human Embryonal Carcinoma Cells

¹ Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York
² Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York; Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York
³ Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, New York
⁴ Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York
⁵ Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York; Division of Neurosurgery, Memorial Sloan-Kettering Cancer Center, New York, New York

^* To whom correspondence should be addressed. E-mail: chagantr{at}mskcc.org.

	Abstract

Human embryonal carcinoma (EC) cell lines exhibit considerable heterogeneity in their levels of pluripotency. Thus, NT2/D1 cells differentiate into neural lineages upon exposure to all-trans retinoic acid (ATRA) and non-neural epithelial lineages upon exposure to Bone Morphogenetic Protein-2 (BMP-2). In contrast, 27X-1 cells differentiate into extra-embryonic endodermal (ExE) cells upon treatment with either morphogen. To understand the molecular basis for the differential responses of the two cell lines, we performed gene expression profiling at the undifferentiated EC cell line state to identify constitutive differences in gene expression. NT2/D1 cells preferentially expressed transcripts associated with neurectodermal development, while 27X-1 cells expressed high levels of transcripts associated with mesendodermal characteristics. We then determined temporal expression profiles of 27X-1 cells during ExE differentiation upon treatment with ATRA and BMP-2 and compared the data with changes in gene expression observed during BMP-2- and ATRA-induced differentiation of NT2/D1 cells. ATRA and BMP-2 induced distinct sets of transcription factors and phenotypic markers in the two EC cell lines underlying distinct lineage choices. While 27X-1 differentiation yielded comprehensive gene expression profiles of parietal endodermal lineages, we were able to use the combined analysis of 27X-1 data with data derived from yolk sac tumors for the identification of transcripts associated with visceral endoderm formation. Our results demonstrate constitutive differences in the levels of pluripotency between NT2/D1 and 27X-1 cells that correlate with linage potential. This study also demonstrates that EC cells can serve as robust models to investigate early lineage choices during both embryonic and extra-embryonic human development.

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