Stem Cells
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First published online August 31, 2006
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2006-0360v1
25/1/78    most recent
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Submitted on June 12, 2006
Accepted on August 23, 2006

Tissue-Specific Stem Cells

Potential of Dental Mesenchymal Cells in Developing Teeth

Hidetoshi Yamazaki 1*, Motokazu Tsuneto 2, Miya Yoshino 2, Ken-Ichi Yamamura 3, Shin-Ichi Hayashi 2

1 Department of Physiology and Regenerative Medicine, Division of Genomics and Regenerative Biology, Institute of Medical Science, Mie University Graduate School of Medicine, Tsu, Japan
2 Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan
3 Division of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan

* To whom correspondence should be addressed. E-mail: yamazaki{at}doc.medic.mie-u.ac.jp.


  Abstract

The tooth, composed of dentin and enamel, develops through epithelium- mesenchyme interactions. Neural crest (NC) cells contribute to the dental mesenchyme in the developing tooth, and differentiate into dentin-secreting odontoblasts. NC cells are known to differentiate into chondrocytes and osteoblasts in the cranio-facial region. However, it is not clear whether the dental mesenchymal cells in the developing tooth possess the potential to differentiate into lineage(s) other than the odontoblast lineage.

In this study, we prepared mesenchymal cells from E13.5 tooth germ and assessed their potential for differentiation in culture. They differentiated into odontoblasts, chondrocyte-like cells and osteoblast-like cells. Their derivation was confirmed by tracing NC-derived cells as LacZ+ cells using P0-Cre/Rosa26R mice. Using the flow cytometry- fluorescent di-{beta}-D-galactosidase system, which makes it possible to detect LacZ+ cells as living cells, cell surface molecules of dental mesenchymal cells were characterized. Large number of LacZ+ NC-derived cells expressed PDGFR{alpha} and integrins. Taken together, these results suggest that NC-derived cells with the potential to differentiate into chondrocyte-like and osteoblast-like cells are present in the developing tooth, and these cells may contribute to tooth organogenesis.

Key Words. Dental mesenchyme, Neural crest-derived cells, Murine tooth, Osteoblast, Chondrocyte, Odontoblast, Tissue-specific stem cells, Cre-loxP system




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[Abstract] [Full Text] [PDF]


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