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Tissue-Specific Stem Cells |
1 Institute of Transfusion Medicine and Immunology, German Red Cross Blood Service of Baden-Württemberg - Hessen, Faculty of Medicine Mannheim, University of Heidelberg, Mannheim, Germany
* To whom correspondence should be addressed. E-mail: k.bieback{at}iti-ma.blutspende.de.
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Abstract |
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Mesenchymal stem cells (MSC) are currently in focus regarding their clinical potential in cell therapy and tissue engineering. However, most isolation and expansion protocols for clinical-scale production of MSC use Fetal Calf Serum (FCS) as supplement which poses a potential risk for infections as well as immunological reactions. In order to find a suitable FCS substitute, we investigated the effects of pooled human AB-Serum (AB-HS) and thrombin-activated Platelet-Rich-Plasma (tPRP) on adipose tissue MSC (AT-MSC) with FCS as the standard control medium.
AT-MSC of 10 donors were cultured under three different conditions: 1) 10% FCS, 2) 10% AB-HS and 3) 10% tPRP. Colony-forming units, cumulative population doubling rates and differentiation capacity towards the adipogenic and osteogenic lineages were assessed as well as immunophenotype.
We demonstrated that AB-HS and tPRP provide a significantly higher proliferative effect on AT-MSC than FCS. In the first 6 passages AB-HS and tPRP MSC exhibited a fold expansion of 66.6 ± 15.7 and 68.1 ± 6.7 respectively, compared to 24.4 ± 0.7 for FCS. Differentiation capacity was preserved throughout long-term culture. Immunophenotype was characteristic for MSC and comparable for all culture conditions with the exception of a distinct CD45/CD14 positive side population for AB-HS and tPRP which tended to diminish with prolonged culture.
We showed that pooled human AB-Serum and thrombin-activated Platelet-Rich-Plasma are alternatives to FCS for AT-MSC. These human sources are better characterized regarding potential infectious threats, while providing a higher proliferation rate and retaining differentiation capacity and mesenchymal stem cell marker expression throughout long-term culture.
Key Words. Mesenchymal stem cells, Fetal calf serum, Platelet-Rich-Plasma, AB-Serum, Adipose tissue
This article has been cited by other articles:
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  F. Mannello and G. A. Tonti Concise Review: No Breakthroughs for Human Mesenchymal and Embryonic Stem Cell Culture: Conditioned Medium, Feeder Layer, or Feeder-Free; Medium with Fetal Calf Serum, Human Serum, or Enriched Plasma; Serum-Free, Serum Replacement Nonconditioned Medium, or Ad Hoc Formula? All That Glitters Is Not Gold! Stem Cells, July 1, 2007; 25(7): 1603 - 1609. [Abstract] [Full Text] [PDF]
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