Stem Cells
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

First published online April 5, 2007
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
2006-0664v1
25/7/1779    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Reprints/Permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duret, C.
Right arrow Articles by Daujat-Chavanieu, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duret, C.
Right arrow Articles by Daujat-Chavanieu, M.
Submitted on October 24, 2006
Accepted on March 28, 2007

Tissue-Specific Stem Cells

ISOLATION, CHARACTERIZATION AND DIFFERENTIATION TO HEPATOCYTE-LIKE CELLS OF NON PARENCHYMAL EPITHELIAL CELLS FROM ADULT HUMAN LIVER

Cédric Duret 1, Sabine Gerbal-Chaloin 1, Jeanne Ramos 2, Jean-Michel Fabre 3, Eric Jacquet 4, Francis Navarro 4, Pierre Blanc 4, Antonio Sa-Cunha 5, Patrick Maurel 1, Martine Daujat-Chavanieu 1*

1 INSERM, U632, 34293 Montpellier, France; Université de Montpellier 1, UMR-S632, Montpellier, France
2 Service d'Anatomie Pathologique, Hôpital Guy de Chauliac, Montpellier, France
3 Service de Chirurgie Digestive II, Hôpital Saint Eloi, Montpellier, France
4 Service Médico-Chirurgical des Maladies de l'Appareil Digestif et Transplantation Hépatique, Hôpital Saint Eloi, Montpellier, France
5 Service de Chirurgie Digestive, Hôpital Haut Lévèque, Pessac, France

* To whom correspondence should be addressed. E-mail: daujat{at}montp.inserm.fr.


  Abstract

Activation and proliferation of human liver progenitor cells has been observed during acute and chronic liver diseases. Our goal was to investigate the presence of these putative progenitors in the liver of patients who underwent lobectomy for various reasons but did not show any hepatic insufficiency. Hepatic lesions were evaluated by histological analysis. Non parenchymal epithelial (NPE) cells were isolated from samples of human liver resections located at a distance from the lesion which motivated the operation and were cultured and characterised. These cells exhibited a marked proliferative potential. They did not express the classical set of stem cell/progenitor markers (Oct-4, Rex-1, {alpha}-fetoprotein, CD90, c-kit, CD34) and were faintly positive for albumin. When cultured at confluence in the presence of hepatocyte growth factor and either epidermal growth factor or fibroblast growth factor-4, they entered a differentiation process towards hepatocytes. Their phenotype was quantitatively compared to that of mature human hepatocytes in primary culture. Differentiated NPE cells expressed albumin, {alpha}1-antitrypsin, fibrinogen, hepato-biliary markers such as cytokeratins 7, 19, and 8/18, liver-enriched transcription factors, and genes characterized by both a fetal (cytochrome P4503A7 and glutathion-S-transferase {pi}) and a mature expression pattern (tyrosine aminotransferase, tryptophan 2,3-dioxygenase, glutathion-S-transferase {alpha} and cytochrome P4503A4). NPE cells could be isolated from the liver of several patients, irrespective of the absence or presence of lesions, and differentiated towards hepatocyte-like cells with an intermediate hepato-biliary and mature/immature phenotype. These cells are likely to represent a resident progenitor population of the adult human liver, even in the absence of hepatic failure.

Key Words. human adult hepatic progenitors, stem cell, oval cell, hepato-biliary cell, differentiation






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
STEM CELLS THE ONCOLOGIST CME ALPHAMED PRESS JOURNALS
http://www.peprotech.com/
Copyright © 2007 by AlphaMed Press.